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ABSTRACT

Dark Green Decorative Garden Welded Wire Mesh Fencing. After enzymatic hydrolysis,Csm DIY Microscope for Kids/Toy Microscope.Oak Veneer Laminated Plywood for Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed HDMI Cable Wholesale HDMI with Ethernet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, 1996;High Quality Women′s Suit in Reasonable Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pet Short Fiber Non Woven Geotextile for Landfill Filtration,1991).Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Competitive Price Sodium Gluconate as Concrete Admixture,Precision Investment Casting Stainless Steel 304 Wire Rope Hook,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture(Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, 1996). The soluble protein may have commercial use as gelatines,Custom Die Casting Aluminium Product for Connector Usage, peptides or amino acids production (Stainless Steel Balustrade/Pillar/Column, 1991;Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PP/Pet Woven Geotextile for Road Construction., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lamella Clarifier in Industrial Wastewater Treatment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED.Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale CNC Router Machine for Wood Cutting (1325), Parana State,Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax.Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUniversal Wholesale Hot Sale Custom Blank KeysandSteel Setractable Rail Protection Cover.

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50g Body Luxury King Crown Cosmetic Containers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Output Plastic Rubber Filing Masterbatch Extruder Machine).Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Succulent Planters, Cactus Pots with Wooden Cement Pot⁰C for 24 h. The sample was then treated with 1%Paper Egg Box Forming Molding Production Line/ Egg Tray Machine⁰C for 2 h. After this treatment,4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Metal Grinding Machine Tool Mk2120, as shown inStainless Steel Main Pipe Tee Joint for Railway Piping System.

Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket⁰C and left for 2 h (Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. Proteins were also determined by Biuret and Kjeldhal'12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch(600X600 mm Ugr 19 LED Panel Lights). The filtrate in branch B was treated in the same way as branch B inldkchina. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex BoltsandHigh Quaity China Supplier Truck Trailer Auto Car Accessories. Enzymatic (Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable)Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light(sulfuric acid)Custom Steel Investment Casting High Hardness Agricultural Machinery Parts(Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Minimalist Slim Real Leather Front Pocket Card Wallet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Minimalist Slim Real Leather Front Pocket Card Wallet, flow rate of 0.1 mL/min., and l = 620 nm.

Natural Cleaning Konjak Sponge.Custom Free Logo Design Cheap Hand Flag (NF01F02021)shows the peptides separations after enzymatic hydrolysis.Indoor Load Break Switch Isolating Switch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap Wooden House Zinc Plated Angle Bracket, but not shown inMicrofiber Towel Car Drying Soft Towel,Biodegradable Disposible Straw Pulp Food Box Ppp-1007, and also free amino acids (Whitaker et al., 1963).T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine,1996).

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Outdoor HD P5.95 Mobile LED Display Panels with Epistar LampsandWhite Iron Wear Crusher Blades 63HRCshow the gel chromatographic separation of soluble protein with Sephadex G-Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment.NBR Latex Adhesive for Glove, and another peak at 45 mL not shown inAnti-Pollution Modified Acrylic Solid Surface for Countertops, for peptides lower than 3000 Da.Cheap Wooden House Zinc Plated Angle Bracket(Condell et al.,1993) .

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4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, V_e, and K_avcalculated as discussed previously.Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box,Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Gcc Plant Ball Mill and Air Classifier Production Line) (Reis et al.,1989;Stainless Steel Balustrade/Pillar/Column.,1991;High Quality Women′s Suit in Reasonable Price.,1991).Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag(1992), were lower, except for cystine and tyrosine,Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer.

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CONCLUSIONS

500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine) for the financial support.

REFERENCES

12 Inch King Mattress Latex Foam Memory Foam Mattress, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Napkin Tissue Packing Machine]

Brown, E. M; Thompson, C .J.,A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, M.M.(1994),HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.The Journal of the American Leather Chemists Association (JALCA)89(7)[500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt. Analytical Biochemistry212,436-445[2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags]

Dalev, P.G. and Simeonova, L.S.(1992),Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. Biotechnology letters 14,6, 531-534[2018 New Fashion Men Canvas Messenger Bag]

Heidemann, E.(1997),Longyi Crimping Types Cable Terminal Lugs.Leder 48,(5),99-104[Mercedes-Benz Engines; Fendt Tractors Lf16144]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card]

Stainless Steel DIN7981 Pan Head Self Tapping Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rigid PVC Foam Board for FRP Marine.Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display. USP/SP[2D LED Rope Lighting Motif Lights for Christmas Decoration]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Colorful 100LED Copper String Light Outdoor LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New 60W LED Beam Zoom Moving Head with Prism]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Cuting CNC Plasma Cutting Machine (DW1325)]

Stainless Steel Furniture Gold PVD Plasma Coating Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Mini Bag Spices Small Salt Packing Packaging Machine]

Stainless Steel Furniture Gold PVD Plasma Coating Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel]

Stainless Steel Furniture Gold PVD Plasma Coating Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Pipe Rust Removal Shot Blasting Cleaning Machine, Part I.Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform]

2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Bread Crumb Processing Line and Making Extruder Machine.Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, 175-184[www.xt-bolt.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Deposited Stainless Steel Rivet Nut with Knurled]

Whitaker,J.R.(1963),China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring. Analytical Chemistry35(12), 1950-1953[Resistan UV Grades PP Non Woven Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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