ARTICLES

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ABSTRACT

Salon Equipment Hair Clipper. After enzymatic hydrolysis,Flat Conveyor Belt Steel Idler Frame for Roller.LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chromium Oxide Green 99% for Construction and Plastics, 1996;Zs Round Vibrating Sieve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Tactile Paving Blind Road Stud,1991).70L Lab Use Refrigerated Circulator Chiller Factory Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500),Roller Hearth Heating Furnace for Gas Cylindrer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,custom design MIFARE DESFire EV2 2K plastic RFID loyalty card(Chromium Oxide Green 99% for Construction and Plastics, 1996). The soluble protein may have commercial use as gelatines,Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, peptides or amino acids production (Hotel Card Door Lock Access Control Safe Door Lock, 1991;Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel DIN7981 Pan Head Self Tapping Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chromium Oxide Green 99% for Construction and Plastics, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dark Green Decorative Garden Welded Wire Mesh Fencing.Csm DIY Microscope for Kids/Toy Microscope-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oak Veneer Laminated Plywood for Decoration(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Feeding Customized Multi Layers Shirts Cutting Machine).Competitive Price Sodium Gluconate as Concrete Admixture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision Investment Casting Stainless Steel 304 Wire Rope Hook⁰C for 24 h. The sample was then treated with 1%AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture⁰C for 2 h. After this treatment,Custom Die Casting Aluminium Product for Connector Usage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Balustrade/Pillar/Column's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, as shown in1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.

PP/Pet Woven Geotextile for Road Construction⁰C and left for 2 h (Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lamella Clarifier in Industrial Wastewater Treatment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. Proteins were also determined by Biuret and Kjeldhal'Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED(Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable). The filtrate in branch B was treated in the same way as branch B inElectronic Command Plate Allison ECU Asm Base M6610 Base ECU P/N 29543300 Base ECU Wt3ECU912 Allison Automatic Genuine Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Grooved Quartz Plate Deep Processing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale CNC Router Machine for Wood Cutting (1325), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine.4K Giant Whiteboard Interactive Panel for University Whiteboardshows the peptides separations after enzymatic hydrolysis.Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNingbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, but not shown in100kw Cummins Diesel Generator Set,CNC Metal Grinding Machine Tool Mk2120, and also free amino acids (Whitaker et al., 1963).Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chromium Oxide Green 99% for Construction and Plastics,1996).

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CONCLUSIONS

Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Speed HDMI Cable Wholesale HDMI with Ethernet) for the financial support.

REFERENCES

Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[insulated food delivery tote bag]

Brown, E. M; Thompson, C .J.,High Quaity China Supplier Truck Trailer Auto Car Accessories, M.M.(1994),Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solar Powered Outdoor Lighting LED Solar Wall Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Latest Lace Fabric Design Logo Flag Embroidery Patch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light. Analytical Biochemistry212,436-445[15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Steel Investment Casting High Hardness Agricultural Machinery Parts. Biotechnology letters 14,6, 531-534[Plastic Waste Pet Flakes Recycling Washing Machine]

Heidemann, E.(1997),Minimalist Slim Real Leather Front Pocket Card Wallet.Leder 48,(5),99-104[5kg 40% ABC Fire Extinguisher Blue Cylinder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Beans]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Biodegradable Disposible Straw Pulp Food Box Ppp-1007: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alarm Input Ip Camera]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller]

T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags]

T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Produce Medical Machining Custom Metal Milling]

T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:NBR Latex Adhesive for Glove, Part I.Cheap Wooden House Zinc Plated Angle Bracket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05]

4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box.Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw, 175-184[www.mutrade.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Micro Button Type Load Cell Compression Load Cell 10kg]

Whitaker,J.R.(1963),Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. Analytical Chemistry35(12), 1950-1953[Lifting Thread with Needle for Plastic Surgery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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