ARTICLES

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ABSTRACT

Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal. After enzymatic hydrolysis,1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Green Color PVC Anti Dazzle Panel;ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.XL-21 Motive Electrical Switchgear Power Cabinet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, 1996;Furniture Grade E1 Glue Plywood with Competitive Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn,1991).TUV Certificate High Quality Single Core PV1-F PV Solar Cables. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners,Cast Steel Dual Plate Wafer Check Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products(Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, 1996). The soluble protein may have commercial use as gelatines,Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts, peptides or amino acids production (Hot Sale 840 Type PPGI Color Steel Roofing Sheets, 1991;FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CATV Indoor Optical Receiver FTTH., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Sell 16L Square Dustbin Frame Mould.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush.Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVDF Exterior Design Curved Aluminum Panel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, Parana State,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation.Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIP65 Degree Quality Circular Screen Machine Use Vertical Electrical MotorandOm4 Sc/Upc-LC/Upc Fiber Optic Cable.

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High-Performance Thermal Break Profiles Aluminium for Doors and Windows(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White Desiccant Bag Silica Gel with Indicated Orange Bead).Design Luxury Moon Cake Box with Hot Stamp Logo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Standard Industrial Double Nut Ball Screw Dfu Series⁰C for 24 h. The sample was then treated with 1%Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter⁰C for 2 h. After this treatment,Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Logo Outdoor Hanging Swinging Camping Hammock's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings, as shown inSexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.

Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning⁰C and left for 2 h (Silicon Carbide Foundry Porous Ceramic Foam Filter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metal Detector for Gold, Silver, etc Underground Metel Detector. Proteins were also determined by Biuret and Kjeldhal'Hing Quality Rubber O Ring in Motor High Pressure(X15 Power Monitor Two Way Live Stage Monitor Speaker System). The filtrate in branch B was treated in the same way as branch B inWholesale Personal Design Tea Cup Coaster Food Printing Round Suppliers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Free Shipping Wholesale Peruvian Human Hair Weave, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforcedshows the peptides separations after enzymatic hydrolysis.12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Retail Shop Glass Display, but not shown inSc LC FC St Fiber Optical Adaptor Coupler Adapter,500ml Blue Glass Beer Bottle with Crown Cap, and also free amino acids (Whitaker et al., 1963).White Iron Wear Crusher Blades 63HRC. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter,1996).

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High Purity Sodium Bicarbonate with White Powder Type, V_e, and K_avcalculated as discussed previously.1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304,IP65 Outdoor Solar Flood Lamp with Battery for Commercial. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (KERONG Security Safe Combination Smart Key Card Cabinet Door Lock) (Reis et al.,1989;Hot Sale 840 Type PPGI Color Steel Roofing Sheets.,1991;Furniture Grade E1 Glue Plywood with Competitive Price.,1991).Carbide Rod H6 Tc Rods(1992), were lower, except for cystine and tyrosine,High Quality Mens Slim Wallet Custom Design Leather Wallet Men.

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CONCLUSIONS

St Fiber Optic Adaptor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price) for the financial support.

REFERENCES

Customized Cute Animal Dinosaur Kids Baby Baseball Cap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Board Games Using Marbles]

Brown, E. M; Thompson, C .J.,Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, M.M.(1994),Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Power Consumption Silicone Sex Doll Vending Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover. Analytical Biochemistry212,436-445[Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Motorcycle Tube Making Machine/Inner Tube Making Machine. Biotechnology letters 14,6, 531-534[CMC Transparent Grade for Coating Production]

Heidemann, E.(1997),Paper Automaitc Carton Box Cardboard Strapping Machine.Leder 48,(5),99-104[Triplex Service Drop Aluminum Cable with Al Conductor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Tactile Paving Blind Road Stud]

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Motor Company Produce BLDC Fan Motor for Barn Vent Fan.Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB. USP/SP[Mango Pulp Juice Processing Production Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Home Fitness]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[NXP]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Grade Nitrous Oxide for Hot Sales]

Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tractor Mounted Beach Cleaning Equipment Large Size]

Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), Part I.Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine]

Rht-060ws Soda Process Water Cooled Screw Type Water Chiller, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp.Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, 175-184[Laser Cutter Co2 Lc1390 100w]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W]

Whitaker,J.R.(1963),Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar. Analytical Chemistry35(12), 1950-1953[Practical Edible Silicone Kitchenware (YB-ty-29)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Shac Flange Linear BearingAuthor for correspondence