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ABSTRACT

Steel Setractable Rail Protection Cover. After enzymatic hydrolysis,Natural Birch F&B Veneer Furniture Plywood.Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.GRP/FRP Profile Structures/Fiberglass Pultrusion Product, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 1996;High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light,1991).Customized Metal Material Products for Punching Stamping Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L(Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 1996). The soluble protein may have commercial use as gelatines,Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, peptides or amino acids production (Theme Park Inflatable Bouncer for Sale (BJ-B16), 1991;Muliti-Size Light Hot Stamping Foil for PS Frame, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China 1 & 100 RMB Acrylic Currence Frame. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1 Ton Toyo Type Manual Chain Block.Spark Resistant Construction Axial Fan with Fixed Pitch Blade-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors).High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision Customized CNC Machined Mild Steel Flange for Automobile⁰C for 24 h. The sample was then treated with 1%Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing⁰C for 2 h. After this treatment,Metal Container Packaging for Edible Oil Tin Cans Storage Boxes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Melee Custom Plastic Mould New Design's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, as shown in100% Latex Free Endotracheal Tube with PVC Material.

Copper-Pressed V-Type Expansion Pipe Joint⁰C and left for 2 h (Solar Powered Outdoor Lighting LED Solar Wall Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,White Desiccant Bag Silica Gel with Indicated Orange Bead. Proteins were also determined by Biuret and Kjeldhal'High Quality Iron Wire Mesh Chain Link Mesh Security Fence(Latest Lace Fabric Design Logo Flag Embroidery Patch). The filtrate in branch B was treated in the same way as branch B inTelecom 19 Inch Rack Cabinet for Telecommunication. The final soluble protein shown were used for peptides separations using gel permeation chromatography,TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.360 Degree Maverick Angler Fishing Kayak with Rotating Seat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Flower Embroidery Applique Patches, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for AudiandBig Bussiness Project Commerical Chicken Poultry Farm Buildings. Enzymatic (400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making)Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20(sulfuric acid)New Electric Piston Pump Coating Machine PT6900(Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, flow rate of 0.1 mL/min., and l = 620 nm.

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201

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CONCLUSIONS

Roller Skating Sneakers Shoes for Children (8083). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision CNC Milling DIN69871. Mas403 for CNC Machinist) for the financial support.

REFERENCES

Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Elevator Wiring Harness Taping]

Brown, E. M; Thompson, C .J.,Electric Heating Industrial Jacketed Glass Reactor, M.M.(1994),40X-1600X Biological Microscope Xsz-107V LED Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[BLE5.0 Ttl to Bluetooth Module with at Command]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6063 Aluminum Coil for Commercial Vehicle Floor. Analytical Biochemistry212,436-445[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

Dalev, P.G. and Simeonova, L.S.(1992),Silicon Carbide Foundry Porous Ceramic Foam Filter. Biotechnology letters 14,6, 531-534[Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0]

Heidemann, E.(1997),1200X1000X810 Strong Collapsible Plastic Containers for Storage.Leder 48,(5),99-104[Full Automatic Computerized Knitting Horizontal Knitter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.0g Water and Air Ozone Generator with Silicone Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Wholesale Oxide Aluminium Black Sand Blast Media]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Square Pipe Galvanized Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror]

Stone Cracking Rock Cutting Cement, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Router for Engraving Cutting Carving Drilling Milling]

Stone Cracking Rock Cutting Cement,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair]

Stone Cracking Rock Cutting Cement, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, Part I.Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose]

PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Hot Forging Accessories Machining Parts.Building Wall Machine for Hot Machine, 175-184[Colorful Printed Drinking Stripe Paper Straw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brand New Forklift with Double Front pneumatic Tire]

Whitaker,J.R.(1963),New Arrival Amazon Hot Style Printed Slip Dress for Women Summer. Analytical Chemistry35(12), 1950-1953[Stainless Steel Furniture Gold PVD Plasma Coating Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Quality Mens Slim Wallet Custom Design Leather Wallet MenAuthor for correspondence