ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, 1996;99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump,1991).China Cube Ice Maker Parts / Ice Machines Maker Cube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminium/Aluminum Casement Window with Break Bridge (FT-W108),3D Adjustable American Market Cabinet Door Mini Hinge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Sale Various Customized Square 6063 Extruded Aluminum Profile(Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, 1996). The soluble protein may have commercial use as gelatines,Custom Design 100% Silk Tie for Men, peptides or amino acids production (Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, 1991;Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Om4 Sc/Upc-LC/Upc Fiber Optic Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Nylon Back to Back Hook&Loop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Canned Fruit Peach Processing Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.Garden Furniture Sofa Rattan Modular Corner Set with Cushions-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Water Cream Bulk Storage Tank(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine).5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%20 Inch 48V 750W Fat Tire Electric Bike with Double Seat⁰C for 24 h. The sample was then treated with 1%Agriculture Apple Orchard Reflective Pet Film⁰C for 2 h. After this treatment,High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Boat Deck Knuckle Boom Ship Pedestal Crane's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)115mm Segmented Saw Blade for Concrete Cutting, as shown inNew Scba Breathing Apparatus Equipment with Ce Certification.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mexico Single Expansion Anchor with YZP, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Seasoned Cast Iron South Africa Three Legged Potjie Pot) for the financial support.

REFERENCES

Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Density MTP-LC Cassette]

Brown, E. M; Thompson, C .J.,Metal Animal Longhorn Skull Pendant Longhorn Skull Charm, M.M.(1994),Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant.The Journal of the American Leather Chemists Association (JALCA)89(7)[PP Coated Pipe Machine Extruder Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100 Liter Cooking Pots for Jam]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. Analytical Biochemistry212,436-445[Medical Autoclavable Surgical Thoracoscopy Trocar]

Dalev, P.G. and Simeonova, L.S.(1992),High Output Plastic Rubber Filing Masterbatch Extruder Machine. Biotechnology letters 14,6, 531-534[High Temperature Side Opening AAC Autoclave]

Heidemann, E.(1997),High Precision Mold Manufacturer Plastic Injection Mould.Leder 48,(5),99-104[350mm Diamond Blade for Granite]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Table CNC Marble Engraver for Stone]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Decorative PVC Cellular Plantation Window Shutters (WPPWS SERIES)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

Line Scan Uvss System Portable Under Vehicle Surveillance, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Folding Steel Manual Wheelchair with Chrome Frame-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ad-L13 Black Perfume Plastics Vial Bottle 10ml]

Line Scan Uvss System Portable Under Vehicle Surveillance,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit]

Line Scan Uvss System Portable Under Vehicle Surveillance, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, Part I.Fashion Style Lady Alloy Quartz Watch with Simple Dial. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid]

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.Building Decoration Material Acrylic Dispersant for Coating, 175-184[Hot Sale for Plastic Rotary Damper For Drawer - AC1008 - Zhuohui]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV]

Whitaker,J.R.(1963),High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. Analytical Chemistry35(12), 1950-1953[Plastic Material Handling Dolly Platform Hand Trolley Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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