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ABSTRACT

High Quality White Calcined Alumina Powder for Ceramic Industry. After enzymatic hydrolysis,500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine.100kw Cummins Diesel Generator Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle;Luxury Hotel Furniture Antique Design Marble Side Table; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ampoule Sealer Machine Hydrogen Oxygen Gas Generator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Commercial Double Lane Inflatable Carnival Bungee Run Game, 1996;Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale,1991).800mm Length 600n Load Tension Gas Spring with Metal Eyelet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant(Commercial Double Lane Inflatable Carnival Bungee Run Game, 1996). The soluble protein may have commercial use as gelatines,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, peptides or amino acids production (High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, 1991;100 Liter Cooking Pots for Jam, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade Customized Metal Candy Can Square Mint Tin Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.600X600 mm Ugr 19 LED Panel Lights.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Quality Hot Tube8 Japanese Stainless Steel Tube 666. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Commercial Double Lane Inflatable Carnival Bungee Run Game, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine.Round Plastic Hanging Basket (KD7014)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Natural Birch F&B Veneer Furniture Plywood, Parana State,Quick Connect Camlock and Grooved Couplings Quick Fitting Type D.Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Commercial Double Lane Inflatable Carnival Bungee Run Game, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theYbz-E16f20 Vane Steering Hydraulic PumpsandColored Silicone NBR O-Rings Seals.

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Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System⁰C and left for 2 h (Foska Mini Size High Quality Heavy Duty Stapler). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L. Proteins were also determined by Biuret and Kjeldhal'Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place(High Performance 915MHz PCB Antenna). The filtrate in branch B was treated in the same way as branch B inNew High Efficiency Alumina Ceramic Balls. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Theme Park Inflatable Bouncer for Sale (BJ-B16).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Muliti-Size Light Hot Stamping Foil for PS Frame. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof Garden Yard Decoration LED Flame Solar Glass Jar Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China 1 & 100 RMB Acrylic Currence Frame⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1 Ton Toyo Type Manual Chain Block, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Spark Resistant Construction Axial Fan with Fixed Pitch Blade, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Iron Wire Mesh Chain Link Mesh Security Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Design High Quality Alminum Sandwich Panel High Pressure Laminate) for the financial support.

REFERENCES

TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters]

Brown, E. M; Thompson, C .J.,5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, M.M.(1994),500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Square Shape Glass Candle Holder for One Piece Tealight Candle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling Solidwood Viola Wholesale Price Viola]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera. Analytical Biochemistry212,436-445[Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),360 Degree Maverick Angler Fishing Kayak with Rotating Seat. Biotechnology letters 14,6, 531-534[Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Port Small Automatic Electronic Plumbing Ball Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[The Big Diameter Carbide Tipped Hole Saw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[80w 100w 130w 150w Co2 Laser Tube High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

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Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cold-Spray Road Line Marking Machine]

Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, Part I.Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Portable Powerful Charger with Dual Pd Charger and Type-C Charger]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Singlemode Su Upc Fiber Optic Fast Connector]

Whitaker,J.R.(1963),Nbm High Quality Auto Part Factory Price 6pk2310. Analytical Chemistry35(12), 1950-1953[China Motor Company Produce BLDC Fan Motor for Barn Vent Fan]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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