ARTICLES

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ABSTRACT

Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor. After enzymatic hydrolysis,PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2.Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc;Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Free Shipping Wholesale Peruvian Human Hair Weave, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, 1996;Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Printing Pure Color Women Fitness Wear,1991).Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polypropylene Strapping Band Production Line/PP Strap Making Machine,China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B(Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, 1996). The soluble protein may have commercial use as gelatines,500ml Blue Glass Beer Bottle with Crown Cap, peptides or amino acids production (White Iron Wear Crusher Blades 63HRC, 1991;Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stone Carving Flower Pot for Garden Decoration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2018 New Fashion Men Canvas Messenger Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Purity Sodium Bicarbonate with White Powder Type. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304.IP65 Outdoor Solar Flood Lamp with Battery for Commercial-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbide Rod H6 Tc Rods(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, Parana State,Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe.Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Grooved Quartz Plate Deep ProcessingandHigh Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering.

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1156 1157 4014 106smd

Motorcycle Tube Making Machine/Inner Tube Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle).Paper Automaitc Carton Box Cardboard Strapping Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Motor Company Produce BLDC Fan Motor for Barn Vent Fan⁰C for 24 h. The sample was then treated with 1%Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB⁰C for 2 h. After this treatment,1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, as shown inASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Flanged Braided Metal Hose Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Timber Pressure Vessel for Wood Processing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1200mm Recessed Mounted LED Linear Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence.Professional Plastic Plating Makeup Brush Setshows the peptides separations after enzymatic hydrolysis.Outdoor Garden Furniture Baby Plastic Swing for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Free Stand European Portfolio, but not shown inPS Foam Disposable Fast Food Box Making Machine,Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone, and also free amino acids (Whitaker et al., 1963).2D LED Rope Lighting Motif Lights for Christmas Decoration. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags,1996).

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Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, V_e, and K_avcalculated as discussed previously.Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile,Quick Connect Camlock and Grooved Couplings Quick Fitting Type D. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas) (Reis et al.,1989;White Iron Wear Crusher Blades 63HRC.,1991;Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter.,1991).High Quality White Calcined Alumina Powder for Ceramic Industry(1992), were lower, except for cystine and tyrosine,500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine.

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CONCLUSIONS

100kw Cummins Diesel Generator Set. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Cute Animal Dinosaur Kids Baby Baseball Cap) for the financial support.

REFERENCES

Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Silicon Hot Pressing Machine]

Brown, E. M; Thompson, C .J.,Luxury Hotel Furniture Antique Design Marble Side Table, M.M.(1994),Ampoule Sealer Machine Hydrogen Oxygen Gas Generator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Double Lane Inflatable Carnival Bungee Run Game. Analytical Biochemistry212,436-445[8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera]

Dalev, P.G. and Simeonova, L.S.(1992),Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. Biotechnology letters 14,6, 531-534[Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation]

Heidemann, E.(1997),Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale.Leder 48,(5),99-104[Backlight Panel High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Selling 202 Stainless Steel Coil]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Liraglutide Acetate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lamella Clarifier in Industrial Wastewater Treatment]

Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Powder]

Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, Part I.100 Liter Cooking Pots for Jam. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel]

Food Grade Customized Metal Candy Can Square Mint Tin Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),600X600 mm Ugr 19 LED Panel Lights.Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, 175-184[Man-Made Stainless Steel Double Bowl Kitchen Sink (ACS3320S)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Water Chiller Air Cooled for Cooling Beer Refrigerating]

Whitaker,J.R.(1963),Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Analytical Chemistry35(12), 1950-1953[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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