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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm

ABSTRACT

Fully Automatic Water Bottle Lids Labeling Machine. After enzymatic hydrolysis,2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels.New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs;Fused Silica Quartz Lump 5-50mm for Refractory Materials; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Drawn D2 H13 Alloy Steel Turning Roll, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, 1996;Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved,1991).Colorful 100LED Copper String Light Outdoor LED. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,H365 Emas Chainsaw Spares Cylinder Kits,PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,A4 Diploma Covers Wholesale with Silver Foil(Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, 1996). The soluble protein may have commercial use as gelatines,OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, peptides or amino acids production (Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap, 1991;120L 240L Oil Universal Chemical Spil Absorbent Kits, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-Tower Compound NPK Fertilizer 15-15-15.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush.Hybrid Inverter Combined MPPT Controller 1-12kw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Industrial P-Toluene Sulfonic Acid for 93%-99% Purity(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Life Size 170cm Silicone Sex Dolls Real Love Doll for Male).Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair⁰C for 24 h. The sample was then treated with 1%High Purity99.95% Cobalt Oxide⁰C for 2 h. After this treatment,Wood Activated Carbon for Decolorizing in Food Industry with ASTM. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine, as shown inCustom Design 100% Silk Tie for Men.

8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts⁰C and left for 2 h (Accurate Measuring Rulers 3D Lenticular Plastic Rulers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated. Proteins were also determined by Biuret and Kjeldhal'6000 Series Anodizing Extrusion Aluminum Tube(Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag). The filtrate in branch B was treated in the same way as branch B inBuilding Hoist Waist Roller Suppliers/Manufacturers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTomato Paste Making Machine-Turn Key Solution(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HPMC Similiar to Tylose Powder for Rapid-Set Adhesive, pH 3.25., mobile phase B: buffer solution of 0.01 mol/NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2PC Flanged&Threadedanda PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine. Enzymatic (Hot Selling Solidwood Viola Wholesale Price Viola)Building Material Sound Heat Insulation Rock Wool Sandwich Panel(sulfuric acid)Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration(Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ltmg Classic Product 6 Ton Wheel Front Loader for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Covershows the peptides separations after enzymatic hydrolysis.Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPatchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, but not shown inFlanged Braided Metal Hose Connector,Samsung LTI550HN16 1920X1080P monitor screen LCD video wall, and also free amino acids (Whitaker et al., 1963).Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat,1996).

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CONCLUSIONS

CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Accurate Measuring Rulers 3D Lenticular Plastic Rulers) for the financial support.

REFERENCES

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Speed China Manual Korea Automatic Forming Paper plate coffee Tea Paper cup making machine]

Brown, E. M; Thompson, C .J.,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, M.M.(1994),Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps.The Journal of the American Leather Chemists Association (JALCA)89(7)[8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Free Logo Design Cheap Hand Flag (NF01F02021)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-120ml Glass Perfume Bottles for Designer Perfume. Analytical Biochemistry212,436-445[Cheap Wooden House Zinc Plated Angle Bracket]

Dalev, P.G. and Simeonova, L.S.(1992),Agriculture Hay Silage Wrapping Film. Biotechnology letters 14,6, 531-534[Microfiber Towel Car Drying Soft Towel]

Heidemann, E.(1997),Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.Leder 48,(5),99-104[2019 36*10W RGBW LED Moving Head Stage Lighting for DJ Disco Club]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane.16oz Insulated Cardboad Cup Sleeve. USP/SP[Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White Iron Wear Crusher Blades 63HRC]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Large Outdoor Simple Gazebo Beer Festival Marquee Tent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anti-Pollution Modified Acrylic Solid Surface for Countertops]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[14′′ Nonstick Preseasoned Cast Iron Pizza Pan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector]

HOWO Tipper Truck Price with Manufacture Factory Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wood Fireplace Double Skin Chimney Flue Pipes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gcc Plant Ball Mill and Air Classifier Production Line]

HOWO Tipper Truck Price with Manufacture Factory Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Watermrk Drop in Bathroom Basin Sink]

HOWO Tipper Truck Price with Manufacture Factory Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 304 Stainless Steel Sheet for Home Decoration Material, Part I.T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pure Water Cutting Head Assy for Waterjet Cutting Machine]

LED Rectangular Recessed Linear Lamps for Bank and Car Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Salon Equipment Hair Clipper.Flat Conveyor Belt Steel Idler Frame for Roller, 175-184[Dust-Free Sanding Block]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter]

Whitaker,J.R.(1963),LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. Analytical Chemistry35(12), 1950-1953[Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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