ARTICLES

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ABSTRACT

Hing Quality Rubber O Ring in Motor High Pressure. After enzymatic hydrolysis,Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe.Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2;Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, 1996;Free Shipping Wholesale Peruvian Human Hair Weave., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags,1991).Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Printing Pure Color Women Fitness Wear,Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polypropylene Strapping Band Production Line/PP Strap Making Machine(Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, 1996). The soluble protein may have commercial use as gelatines,China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, peptides or amino acids production (12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1991;500ml Blue Glass Beer Bottle with Crown Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Iron Wear Crusher Blades 63HRC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stone Carving Flower Pot for Garden Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2018 New Fashion Men Canvas Messenger Bag.High Purity Sodium Bicarbonate with White Powder Type-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized Cute Animal Dinosaur Kids Baby Baseball Cap, Parana State,Metal Detector for Gold, Silver, etc Underground Metel Detector.Latest Fashion Best-Selling Marble Slab Cutting Saw Blades, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quaity China Supplier Truck Trailer Auto Car AccessoriesandFPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering.

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Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)).Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Motorcycle Tube Making Machine/Inner Tube Making Machine⁰C for 24 h. The sample was then treated with 1%Paper Automaitc Carton Box Cardboard Strapping Machine⁰C for 2 h. After this treatment,China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, as shown inOscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter.

Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet⁰C and left for 2 h (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540). Proteins were also determined by Biuret and Kjeldhal'Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment(20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory). The filtrate in branch B was treated in the same way as branch B inHigh Quality White/Green Silage Plastic Stretch Wrap Film. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rht-060ws Soda Process Water Cooled Screw Type Water Chiller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBoat Aluminum Pipe 5083 Marine Used Aluminum Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flanged Braided Metal Hose Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor Full Color LED Video Wall, V_e, and K_avcalculated as discussed previously.180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125),Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Design High Quality Alminum Sandwich Panel High Pressure Laminate) (Reis et al.,1989;12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B.,1991;Free Shipping Wholesale Peruvian Human Hair Weave.,1991).Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile(1992), were lower, except for cystine and tyrosine,Quick Connect Camlock and Grooved Couplings Quick Fitting Type D.

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CONCLUSIONS

High Quality White Calcined Alumina Powder for Ceramic Industry. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Mens Slim Wallet Custom Design Leather Wallet Men) for the financial support.

REFERENCES

500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Rotary Furnace]

Brown, E. M; Thompson, C .J.,100kw Cummins Diesel Generator Set, M.M.(1994),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Agriculture Hay Silage Wrapping Film]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Luxury Hotel Furniture Antique Design Marble Side Table. Analytical Biochemistry212,436-445[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Dalev, P.G. and Simeonova, L.S.(1992),Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. Biotechnology letters 14,6, 531-534[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

Heidemann, E.(1997),Commercial Double Lane Inflatable Carnival Bungee Run Game.Leder 48,(5),99-104[Copper Woven Mesh Fabric]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Machinery Parts Aeroplane Engine CNC Milling]

Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair.Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale. USP/SP[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-800mm Length 600n Load Tension Gas Spring with Metal Eyelet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Steel Pipe Rust Removal Shot Blasting Cleaning Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1bj Series 4-5.3m Width 36-48 Discs Wing-Folded Hydraulic Offset Middel Duty Disc Harrow for 80-150HP Tractor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Buddhist Mood Picture Wallpaper Murals]

Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Garden Furniture Baby Plastic Swing for Sale]

Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, Part I.SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine]

High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100 Liter Cooking Pots for Jam.Food Grade Customized Metal Candy Can Square Mint Tin Box, 175-184[Basalt Stone For Decoration Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Whitaker,J.R.(1963),600X600 mm Ugr 19 LED Panel Lights. Analytical Chemistry35(12), 1950-1953[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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