ARTICLES

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ABSTRACT

Baby Clothes Wear Fleece Baby Bunting Onesie Jacket. After enzymatic hydrolysis,Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.Reflective Sheeting Material for Licence Plate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industrial Grade Nitrous Oxide for Hot Sales;Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fire Alarm Detector 24VDC Reflective Beam Detector, 1996;Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Mini Bag Spices Small Salt Packing Packaging Machine,1991).No 55 Steel Wire Cross Type Razor Barbed Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4V330-10 Air Compressor Solenoid Industrial Valve,Professional Various Standard Hot Sale Rotary Lab Single Round Punch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(Fire Alarm Detector 24VDC Reflective Beam Detector, 1996). The soluble protein may have commercial use as gelatines,Light Blue Color Glass Drink Bottle for Beer, peptides or amino acids production (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, 1991;IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof PVDF Aluminum Curtain Wall Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fire Alarm Detector 24VDC Reflective Beam Detector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Round Modern Furniture Leisure Sofa for Promotion.Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sany Hg3333m Climbing Placing Boom(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, Parana State,Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.CNC Machined Power Transmission Shaft Part, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fire Alarm Detector 24VDC Reflective Beam Detector, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSSAW Steel Pipe API 5L Psl2 with Stainless Steel SheetandFully Automatic Water Bottle Lids Labeling Machine.

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Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Screw/Countersunk Head Screw 6 Ribs with Wing).Good Price Channel Steel Used for Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator⁰C for 24 h. The sample was then treated with 1%Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology⁰C for 2 h. After this treatment,Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Direct Sale Patent up to Down Rubber Tile Curing Press's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, as shown inHPMC Hydroxy Propyl Methyl Cellulose Additives.

Real Leather Wallet Mens Designer Leather Wallets⁰C and left for 2 h (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Port Small Automatic Electronic Plumbing Ball Valve. Proteins were also determined by Biuret and Kjeldhal'Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(Paper Egg Box Forming Molding Production Line/ Egg Tray Machine). The filtrate in branch B was treated in the same way as branch B inAllen Cap Bolt Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cleanroom White PVC Dotted Anti-Static Gloves.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMarine Solas Adhesive Reflective Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ASTM A240 304 316L No. 1 Stainless Steel Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12V 24V 220V Digital Temperature Controller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand RopeandBathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. Enzymatic (25kg Fertilizer Belt Packing Machine for Special Materials)Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses(sulfuric acid)Rotor High Output Electric 120 Watt Motor(Fire Alarm Detector 24VDC Reflective Beam Detector, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, flow rate of 0.1 mL/min., and l = 620 nm.

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.125kHz RFID Card 13.56MHz Proximity Card Blank Thin Cardshows the peptides separations after enzymatic hydrolysis.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetric Female Cone Hydraulic Hose Fitting, but not shown inGreen Color PVC Anti Dazzle Panel,Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, and also free amino acids (Whitaker et al., 1963).Rpl Straight Socket and Plugs Male Thread. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fire Alarm Detector 24VDC Reflective Beam Detector,1996).

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2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, V_e, and K_avcalculated as discussed previously.Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape,8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hybrid Inverter Combined MPPT Controller 1-12kw) (Reis et al.,1989;Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.,1991;Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle.,1991).WPC Wood Plastic Board Equipment Extruder Machine(1992), were lower, except for cystine and tyrosine,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS.

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CONCLUSIONS

Neoprene Elbow Support. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Sides Clear White Frosted Acrylic Sheet) for the financial support.

REFERENCES

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medical Disposable Oxygen Face Mask with Tube]

Brown, E. M; Thompson, C .J.,CNC Produce Medical Machining Custom Metal Milling, M.M.(1994),Multi Sensor Nilitary Surveillance Thermal Imaging Security camera.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion Women Hair Accessories Hair Bow Headband for Girls]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Metal Stamping Welding Spare Parts for Farming. Analytical Biochemistry212,436-445[15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Biotechnology letters 14,6, 531-534[ Cnice Brand Toothpaste with Oral Refreshing]

Heidemann, E.(1997),Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation.Leder 48,(5),99-104[Aluminum CNC Prototype Parts Precision Machining]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Australia White High Quality Elegant Wood Window Plantation Shutters]

Single Core Solid Copper Conductor Rg Coaxial Cable, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),275ml Frosted Clear Glass Drinking Bottle for Juice.EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. USP/SP[China Shipping Logistics to Stockholm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cleanroom White PVC Dotted Anti-Static Gloves]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12v Usb Circuit Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Metal Material Products for Punching Stamping Part]

Bolts and Nuts Set Brass Fasteners Double Hex Bolt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Direct Sales Kids Boy Cotton Children T Shirt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion High Quality Head Layer Genuine Leather Men′s Wallet]

Bolts and Nuts Set Brass Fasteners Double Hex Bolt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price]

Bolts and Nuts Set Brass Fasteners Double Hex Bolt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, Part I.15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Universal Mirco Forklift Car Bus Truck Seats Switch Sensors]

Light Blue PVC Plastic Sheet and PVC Film, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy.Metric Female Cone Hydraulic Hose Fitting, 175-184[China Acupressure Mat Ben Greenfield]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring]

Whitaker,J.R.(1963),Hot Sale Dental Material Dental PMMA Block From China. Analytical Chemistry35(12), 1950-1953[Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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