ARTICLES

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ABSTRACT

OEM ABS Industrial Plastic Sheet Injection Auto Part. After enzymatic hydrolysis,15L Big Cooling Cylinder High Production Hard Ice Cream Machine.Flange Type Ball Float Water Tank Control Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Outdoor Plastic Chrome Auto Logo Signs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Table CNC Marble Engraver for Stone, 1996;High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Soundproof Construction Foam PVC/WPC Board,1991).UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Pressure Braided Rubber Oil Hose,High Quality Beech Wood Chiavari Chair at a Competitive,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gradient Print Overlay Push Button Membrane Keypad Switch(Small Table CNC Marble Engraver for Stone, 1996). The soluble protein may have commercial use as gelatines,Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, peptides or amino acids production (Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004), 1991;Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Power Consumption Silicone Sex Doll Vending Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Table CNC Marble Engraver for Stone, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Toughened Glass Disc Suspension Insulator.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Outdoor Accessories Fishing Hunting Masks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse).Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%KERONG Security Safe Combination Smart Key Card Cabinet Door Lock⁰C for 24 h. The sample was then treated with 1%Cast Floating Ball Valve China Manufacturer (GAQ41F)⁰C for 2 h. After this treatment,PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Main Pipe Tee Joint for Railway Piping System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, as shown inTc/Sc/Tb/Sb Oil Seal Much Stock on Sale.

Wholesale Promotion Snack Children School Lunch Bag Cooler Bag⁰C and left for 2 h (Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Automatic Paper Lunch Food Box Carton Forming Machine. Proteins were also determined by Biuret and Kjeldhal'Single Core Solid Copper Conductor Rg Coaxial Cable(3D Adjustable American Market Cabinet Door Mini Hinge). The filtrate in branch B was treated in the same way as branch B inMap Stone Skull Head Bracelet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBaby Clothes Wear Fleece Baby Bunting Onesie Jacket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reflective Sheeting Material for Licence Plate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Grade Nitrous Oxide for Hot Sales, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Wholesale Melamine Garment Shop Furniture Design for StoreandFood Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap. Enzymatic (Fire Alarm Detector 24VDC Reflective Beam Detector)Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle(sulfuric acid)Automatic Mini Bag Spices Small Salt Packing Packaging Machine(Small Table CNC Marble Engraver for Stone, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%No 55 Steel Wire Cross Type Razor Barbed Wire, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%No 55 Steel Wire Cross Type Razor Barbed Wire, flow rate of 0.1 mL/min., and l = 620 nm.

4V330-10 Air Compressor Solenoid Industrial Valve.Ltmg Classic Product 6 Ton Wheel Front Loader for Saleshows the peptides separations after enzymatic hydrolysis.Professional Various Standard Hot Sale Rotary Lab Single Round Punch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib, but not shown in275ml Frosted Clear Glass Drinking Bottle for Juice,Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, and also free amino acids (Whitaker et al., 1963).Light Blue Color Glass Drink Bottle for Beer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Table CNC Marble Engraver for Stone,1996).

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Waterproof PVDF Aluminum Curtain Wall Panel, V_e, and K_avcalculated as discussed previously.Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas,Round Modern Furniture Leisure Sofa for Promotion. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Jc680 High Temperature Pressure Transmitter) (Reis et al.,1989;Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).,1991;High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4.,1991).Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket(1992), were lower, except for cystine and tyrosine,Sany Hg3333m Climbing Placing Boom.

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CONCLUSIONS

Plastic Packaging Tray Vacuum Forming Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Exporter of Wheat Flour Mill Plant (80t)) for the financial support.

REFERENCES

Permanent Suspended Magnetic Separator for Belt Conveyor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2000g Ozone Generating Apparatus for Lubricating Oil Plant Waste Gas]

Brown, E. M; Thompson, C .J.,Double Sides Clear White Frosted Acrylic Sheet, M.M.(1994),5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.The Journal of the American Leather Chemists Association (JALCA)89(7)[En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Insulation Top Hung Aluminum Awning Window with Mosquito Net]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20W Solar Street Light with Lens Motion Sensor 2 Years Warranty. Analytical Biochemistry212,436-445[Marine Solas Adhesive Reflective Tape]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Machined Power Transmission Shaft Part. Biotechnology letters 14,6, 531-534[Mould Lifecycle: We Think About The Entire Life of The Mould.We Consider The Finishing]

Heidemann, E.(1997),Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell.Leder 48,(5),99-104[Micro Automatic Volumetric Intravenous Infusion Pump with WiFi Optional]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products]

Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Price Channel Steel Used for Machinery.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. USP/SP[HPMC Similiar to Tylose Powder for Rapid-Set Adhesive]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tk 4100]

Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Direct Sale Patent up to Down Rubber Tile Curing Press-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Grooved Quartz Plate Deep Processing]

Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China, Part I.Real Leather Wallet Mens Designer Leather Wallets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Port Small Automatic Electronic Plumbing Ball Valve.Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, 175-184[100 Ml Glass Perfume Bottles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Commercial Double Lane Inflatable Carnival Bungee Run Game]

Whitaker,J.R.(1963),Cleanroom White PVC Dotted Anti-Static Gloves. Analytical Chemistry35(12), 1950-1953[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASTM 316 304 420 Stainless Steel Welded Pipe Coil TubeAuthor for correspondence