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ABSTRACT

No 55 Steel Wire Cross Type Razor Barbed Wire. After enzymatic hydrolysis,4V330-10 Air Compressor Solenoid Industrial Valve.Professional Various Standard Hot Sale Rotary Lab Single Round Punch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute;Light Blue Color Glass Drink Bottle for Beer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, 1996;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof PVDF Aluminum Curtain Wall Panel,1991).Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Round Modern Furniture Leisure Sofa for Promotion,Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sany Hg3333m Climbing Placing Boom(IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, 1996). The soluble protein may have commercial use as gelatines,Plastic Packaging Tray Vacuum Forming Machine, peptides or amino acids production (Permanent Suspended Magnetic Separator for Belt Conveyor, 1991;Double Sides Clear White Frosted Acrylic Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Machined Power Transmission Shaft Part. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell.Good Price Channel Steel Used for Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Full Port Small Automatic Electronic Plumbing Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator).Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cleanroom White PVC Dotted Anti-Static Gloves⁰C for 24 h. The sample was then treated with 1%High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail⁰C for 2 h. After this treatment,Marine Solas Adhesive Reflective Tape. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, as shown inLaminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor.

ASTM A240 304 316L No. 1 Stainless Steel Sheet⁰C and left for 2 h (Custom Metal Stamping Welding Spare Parts for Farming). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.12V 24V 220V Digital Temperature Controller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. Proteins were also determined by Biuret and Kjeldhal'25kg Fertilizer Belt Packing Machine for Special Materials(200m Diesel Oil Hydraulic Water Well Drilling Machine). The filtrate in branch B was treated in the same way as branch B inm.hotonmc.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rotor High Output Electric 120 Watt Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rpl Straight Socket and Plugs Male Thread, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera.Hydraulic Press Corrugated Cold Roll Automatically Forming Machineshows the peptides separations after enzymatic hydrolysis.WPC Wood Plastic Board Equipment Extruder Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3D Creative Design Metal Medal Promotion Gift of Sport, but not shown inFashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, and also free amino acids (Whitaker et al., 1963).Neoprene Elbow Support. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor,1996).

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Custom Metal Stamping Welding Spare Parts for Farming, V_e, and K_avcalculated as discussed previously.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hsac Shac Linear Guideway) (Reis et al.,1989;Permanent Suspended Magnetic Separator for Belt Conveyor.,1991;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.,1991).275ml Frosted Clear Glass Drinking Bottle for Juice(1992), were lower, except for cystine and tyrosine,EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price.

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CONCLUSIONS

Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Direct Sale Patent up to Down Rubber Tile Curing Press) for the financial support.

REFERENCES

Bolts and Nuts Set Brass Fasteners Double Hex Bolt, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ASTM HDG ERW Round Steel Pipe]

Brown, E. M; Thompson, C .J.,Factory Direct Sales Kids Boy Cotton Children T Shirt, M.M.(1994),Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Outdoor Simple Gazebo Beer Festival Marquee Tent]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. Analytical Biochemistry212,436-445[High Quality Couple Watches Steel Material Gift Watch OEM in China]

Dalev, P.G. and Simeonova, L.S.(1992),Light Blue PVC Plastic Sheet and PVC Film. Biotechnology letters 14,6, 531-534[Rigid PVC Foam Board for FRP Marine]

Heidemann, E.(1997),Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy.Leder 48,(5),99-104[Cnc Turret Punch Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Mini LED Flood Light SMD Flood Light 50W]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Waste Pet Flakes Recycling Washing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Tensisle Strength Tissue Paper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Women Flannel Shirt Plaid Flannel Shirts For Men]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl]

Deposited Stainless Steel Rivet Nut with Knurled, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Stainless Steel Mechanical Filter Hosing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring]

Deposited Stainless Steel Rivet Nut with Knurled,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Big Bussiness Project Commerical Chicken Poultry Farm Buildings]

Deposited Stainless Steel Rivet Nut with Knurled, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Paper New Year Greeting Cards Wedding Invitations Cards, Part I.Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card]

BLE5.0 Ttl to Bluetooth Module with at Command, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle.Professional Customization Screen Printing Machine for Porous Ceramic, 175-184[Lightweight Main Tee Roll Forming Machine Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove]

Whitaker,J.R.(1963),Fea040b New Arrival Airplane Seat Belt Extender. Analytical Chemistry35(12), 1950-1953[High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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