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ABSTRACT

10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents. After enzymatic hydrolysis,New Design High Quality Alminum Sandwich Panel High Pressure Laminate.Steel Setractable Rail Protection Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Tire Repair Tool Kit, Tire Repair Strip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Performance 915MHz PCB Antenna, 1996;GRP/FRP Profile Structures/Fiberglass Pultrusion Product., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel,1991).High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light,Customized Metal Material Products for Punching Stamping Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System(High Performance 915MHz PCB Antenna, 1996). The soluble protein may have commercial use as gelatines,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller, peptides or amino acids production (Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, 1991;Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Theme Park Inflatable Bouncer for Sale (BJ-B16)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Muliti-Size Light Hot Stamping Foil for PS Frame.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Performance 915MHz PCB Antenna, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court.China 1 & 100 RMB Acrylic Currence Frame-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1 Ton Toyo Type Manual Chain Block(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Building Material of Granite Ceramic Wall Tile for Wall Decoration(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion).3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box⁰C for 24 h. The sample was then treated with 1%High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop⁰C for 2 h. After this treatment,Precision Customized CNC Machined Mild Steel Flange for Automobile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Container Packaging for Edible Oil Tin Cans Storage Boxes, as shown inDie Cast Iron Worm Gear Speed Reduce Gearbox.

Melee Custom Plastic Mould New Design⁰C and left for 2 h (High Quality Flange Dismantling Joint). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Copper-Pressed V-Type Expansion Pipe Joint. Proteins were also determined by Biuret and Kjeldhal'Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber(HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance). The filtrate in branch B was treated in the same way as branch B inSealing Kraft Paper Tape. The final soluble protein shown were used for peptides separations using gel permeation chromatography,White Desiccant Bag Silica Gel with Indicated Orange Bead.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Iron Wire Mesh Chain Link Mesh Security Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera, pH 3.25., mobile phase B: buffer solution of 0.01 mol/360 Degree Maverick Angler Fishing Kayak with Rotating Seat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home) for the financial support.

REFERENCES

Singlemode Su Upc Fiber Optic Fast Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chocolate Coated Protein Bar Machine High Quality Supplier]

Brown, E. M; Thompson, C .J.,Roller Skating Sneakers Shoes for Children (8083), M.M.(1994),Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Iron Wire Mesh Chain Link Mesh Security Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Heating Industrial Jacketed Glass Reactor. Analytical Biochemistry212,436-445[High Quality Brushless DC Electric Motor for Industrial Deduster]

Dalev, P.G. and Simeonova, L.S.(1992),40X-1600X Biological Microscope Xsz-107V LED Light. Biotechnology letters 14,6, 531-534[Biogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment]

Heidemann, E.(1997),6063 Aluminum Coil for Commercial Vehicle Floor.Leder 48,(5),99-104[Wood Sawdust Pellet Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Men Women Spring Hinges Reading Glasses Classic Real Wood Finish Readers +1.0~+4.0 Plastic Eyeglasses]

Round Plastic Hanging Basket (KD7014), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Silicon Carbide Foundry Porous Ceramic Foam Filter.1200X1000X810 Strong Collapsible Plastic Containers for Storage. USP/SP[Roller Hearth Heating Furnace for Gas Cylindrer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CMP No Nc Metal Switch Black 25mm Illuminated Push Button]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Construction in Poultry House with Good Quality and Installation: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Pulling Force Rotatable Rare Earth Crane Magnet Hook]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Folded]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar]

Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car]

Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet]

Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stone Cracking Rock Cutting Cement, Part I.8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Free Shipping Wholesale Peruvian Human Hair Weave]

PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, 175-184[Plastic Industry Mold Temperature Controller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car]

Whitaker,J.R.(1963),Aluminum Hot Forging Accessories Machining Parts. Analytical Chemistry35(12), 1950-1953[Mobile Hydraulic Concrete Placing Boom]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber CuttingAuthor for correspondence