ARTICLES

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ABSTRACT

BLE5.0 Ttl to Bluetooth Module with at Command. After enzymatic hydrolysis,Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle.Professional Customization Screen Printing Machine for Porous Ceramic. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Square Shape Glass Candle Holder for One Piece Tealight Candle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, 1996;Medical Autoclavable Surgical Thoracoscopy Trocar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge,1991).Easy Foldable Kids Walking Chair Toys Baby Walker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men V-Neck Longline T-Shirt with Color Block Ribbed Trims,Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve(1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, 1996). The soluble protein may have commercial use as gelatines,Outdoor Multifunctional Solar Power Flashlight (SYSG-517), peptides or amino acids production (Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product, 1991;Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FC Bare Fiber Optic Adapter for Network.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cattle Corpse Cremation Burning Stove.L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Micro Button Type Load Cell Compression Load Cell 10kg(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Price of a Retail Supermarket Push Cart for Sale).Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Interlocking Precast Plastic Concrete Paver Molds for Sale⁰C for 24 h. The sample was then treated with 1%24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum⁰C for 2 h. After this treatment,900-1800MHz GSM PCB Antenna, 3m Adhesive GSM Built-in PCB Antenna. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Flange Housing Ucf310-115 for Electric Hand Tools, as shown in3bbl Brite Servicing Tank Beer Brewery Equipment Unitank.

Two Sided Lighted Vanity Mirror Folding 1X and 5X or 10X LED Makeup Mirrors⁰C and left for 2 h (130kw 7 Degrees Scroll Type Industrial Water Chiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester. Proteins were also determined by Biuret and Kjeldhal'Tct Circular Saw Blade &Diamond Saw Blade(Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat). The filtrate in branch B was treated in the same way as branch B in0144) - Pedicure Supplies. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lifting Thread with Needle for Plastic Surgery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMethyl Cyclohexane Methylcyclohexane, Organic Chemicsls(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fudan Compatible Card 1k RFID Card 13.56MHz⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biodegradable Food Packaging Take out Food Boxes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Foska Mini Size High Quality Heavy Duty Stapler.Sinotruk-HOWO 6X4 Tractor Truckshows the peptides separations after enzymatic hydrolysis.5.5X19mm Hex Head Self Tapping Screws with Washer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine, but not shown inWaterproof Garden Yard Decoration LED Flame Solar Glass Jar Light,Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose, and also free amino acids (Whitaker et al., 1963).Low Volume Hard Tool Kit Thermoplastic Polymer Injection Molding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box,1996).

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CONCLUSIONS

5 Star European Antique Hotel Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive) for the financial support.

REFERENCES

4.0MP Ahd Tvi CVBS HD CCTV Camera Module, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Super Clear Bopp Packing Tape For Cartons]

Brown, E. M; Thompson, C .J.,Active Demand Plastic 65 Liter Laundry Basket Mould, M.M.(1994),Organic Fertilizer Compost Turning Machine, Compost Turner Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[H365 Emas Chainsaw Spares Cylinder Kits]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Insulation Top Hung Aluminum Awning Window with Mosquito Net. Analytical Biochemistry212,436-445[IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash]

Dalev, P.G. and Simeonova, L.S.(1992),4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger. Biotechnology letters 14,6, 531-534[High Purity99.95% Cobalt Oxide]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Band Cable Ties Wire Strap Steel Banding: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Road Construction Non Woven Needle Punched PP Non Woven Geotextile]

Acid Water Pulse Output Liquid Orifice Type Flow Meter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700]

Acid Water Pulse Output Liquid Orifice Type Flow Meter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Acid Water Pulse Output Liquid Orifice Type Flow Meter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, Part I.15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[custom design MIFARE DESFire EV2 2K plastic RFID loyalty card]

Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production.Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen, 175-184[Paint Spray Booth Painting Equipment for Car Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dark Green Decorative Garden Welded Wire Mesh Fencing]

Whitaker,J.R.(1963),High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing. Analytical Chemistry35(12), 1950-1953[Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

8011 Aluminum Foil for Cap UsedAuthor for correspondence