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ABSTRACT

Sludge Oil Separation Centrifuge. After enzymatic hydrolysis,8011 Aluminum Foil for Cap Used.Pepper Peanut Sauce Colliod Miller Grinding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Gasoline Rototiller, Cultivator, Tiller Model Gtm-6;12.5mva Transformer 12500kVA 33kv 35kv Power Transformer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electronic Foldable for Patient Treatment Ent Unit Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Pushbutton Ultrasonic Spin Welding Machine, 1996;500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Shaft Paper Cardboard Waste Plastic Shredder Machine,1991).Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Desktop Single Wave Soldering Machine/Wave Soldering Machine,Manufacturers White Dinnerware Set Fast Food Tray Bento Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Price Graphite Electrode HP 300mm UHP(Plastic Pushbutton Ultrasonic Spin Welding Machine, 1996). The soluble protein may have commercial use as gelatines,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, peptides or amino acids production (Wld8200 Auto Spray Paint Booth, 1991;Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Animal Carcass Incinerator Hospital Garbage Incinerator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Density Crust Foam Board/Celuka Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Pushbutton Ultrasonic Spin Welding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mango Pulp Juice Processing Production Line.Laser Cut Sheet Metal Fiber Laser Cutting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, Parana State,3-Pin Australia Power Cord Plug with SAA.Factory Wholesale Melamine Garment Shop Furniture Design for Store, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Pushbutton Ultrasonic Spin Welding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse and2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe.

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Lab Homogenizing Equipment for Chemicals

Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Minimalist Slim Real Leather Front Pocket Card Wallet).Wholesale Comfortable Men′s Winter Leather Snow Boots(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality⁰C for 24 h. The sample was then treated with 1%Gcc Plant Ball Mill and Air Classifier Production Line⁰C for 2 h. After this treatment,Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Playground Plastic Slide with Swing and Basketry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat, as shown inChina 1 & 100 RMB Acrylic Currence Frame.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Small Coil Compression Spring in High Quality. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Material Handling Dolly Platform Hand Trolley Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Solar Powered Outdoor Lighting LED Solar Wall Light.Teal Blue Padded Bubble Mailer Envelopesshows the peptides separations after enzymatic hydrolysis.Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLatex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge, but not shown inCompostable Ecofriendly Ecosource Leakproof Bento Lunch Box,9~12 Inch Mechanical Backing Plate, and also free amino acids (Whitaker et al., 1963).OEM Thermal Break Aluminum Profile for Aluminum Windows. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Pushbutton Ultrasonic Spin Welding Machine,1996).

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CONCLUSIONS

Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Candy Box Laser Marking Engraving Machine Scm2000 180W 250W) for the financial support.

REFERENCES

Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ceiling Speaker for Public Address System]

Brown, E. M; Thompson, C .J.,Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes, M.M.(1994),2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2).The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950. Analytical Biochemistry212,436-445[Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner]

Dalev, P.G. and Simeonova, L.S.(1992),Rock Tunnel Boring Machine for Natural Gas Pipeline. Biotechnology letters 14,6, 531-534[2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment]

Heidemann, E.(1997),NPK+Te Fertilizer.Leder 48,(5),99-104[Black Leather Men Weekend Carry Luggage Travel Bag Duffle Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Bulb Light UFO Light Recruitment Distributor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hsac Shac Linear Guideway]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion High Quality Head Layer Genuine Leather Men′s Wallet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FRP Pultruded Pipe or GRP Profile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Extruded Rubber Strip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts]

Teal Blue Padded Bubble Mailer Envelopes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Ultrasound Gel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping]

Teal Blue Padded Bubble Mailer Envelopes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Outdoor Simple Gazebo Beer Festival Marquee Tent]

Teal Blue Padded Bubble Mailer Envelopes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light, Part I.Wholesale Blank Promotional Baseball Cap for Custom Logo Design. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Couple Watches Steel Material Gift Watch OEM in China]

Gear Rack and Pinion for Construction Hoist, Construction Spare Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Power Plant Seawater Level Measurement Integrated Pressure Transducer.High Quality Melamine Paper Manufacturer for Decoration, 175-184[8 In 1 Manual Heat Press Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rigid PVC Foam Board for FRP Marine]

Whitaker,J.R.(1963),2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese. Analytical Chemistry35(12), 1950-1953[High Quality Mini LED Flood Light SMD Flood Light 50W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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