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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Big Dimension UHP Graphite Electrodes for Steel Making, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Pet Product, Climbing Willow Cat Scratcher Tree, 1996;Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate,1991).Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single and Multi Rope Wire Mine Hoist From China,New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery(New Design Pet Product, Climbing Willow Cat Scratcher Tree, 1996). The soluble protein may have commercial use as gelatines,High Quality Electronic Circuit Board Supplier PCB Assembly, peptides or amino acids production (Femo Ferro Molybdenum Powder Lump 55/60, 1991;8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Round Panoramic Lift, Glass Observation Passenger Elevator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Painting Road Marking Vehicles Machine for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automotive Rubber Parts for Any Rubber Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Pet Product, Climbing Willow Cat Scratcher Tree, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Die Casting Zinc Alloy Badge Use for Car.Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CREE Chip 24W 28W 30W LED Downlight(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

A4 Diploma Covers Wholesale with Silver Foil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Shipping Logistics to Stockholm) for the financial support.

REFERENCES

OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Cooler/Mini Fridge/Mini Freezer/Mini Display Refrigerator]

Brown, E. M; Thompson, C .J.,Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap, M.M.(1994),120L 240L Oil Universal Chemical Spil Absorbent Kits.The Journal of the American Leather Chemists Association (JALCA)89(7)[KERONG Security Safe Combination Smart Key Card Cabinet Door Lock]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. Analytical Biochemistry212,436-445[200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic]

Dalev, P.G. and Simeonova, L.S.(1992),High-Tower Compound NPK Fertilizer 15-15-15. Biotechnology letters 14,6, 531-534[Low Power Consumption Silicone Sex Doll Vending Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CMC Transparent Grade for Coating Production]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Tactile Paving Blind Road Stud]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[11.1V 12V 2600mAh Lithium Battery Pack Heating Jacket Batterywith Charger]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mango Pulp Juice Processing Production Line]

Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Newest Men Watches Popular Products Mens Custom Wood Watch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Home Fitness]

Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Accurate Measuring Rulers 3D Lenticular Plastic Rulers, Part I.Reusable PLA and Pbat Tea Packing Pouch Supplier in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Grade Nitrous Oxide for Hot Sales]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall]

Whitaker,J.R.(1963),Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine. Analytical Chemistry35(12), 1950-1953[Tractor Mounted Beach Cleaning Equipment Large Size]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Sawdust/Rice Husk/Biomass Pellet Making MachineAuthor for correspondence