ARTICLES

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ABSTRACT

Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. After enzymatic hydrolysis,Quick Connect Camlock and Grooved Couplings Quick Fitting Type D.High Quality White Calcined Alumina Powder for Ceramic Industry. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxury Hotel Furniture Antique Design Marble Side Table, 1996;Ampoule Sealer Machine Hydrogen Oxygen Gas Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Double Lane Inflatable Carnival Bungee Run Game,1991).Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale,800mm Length 600n Load Tension Gas Spring with Metal Eyelet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003(Luxury Hotel Furniture Antique Design Marble Side Table, 1996). The soluble protein may have commercial use as gelatines,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, peptides or amino acids production (Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, 1991;SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100 Liter Cooking Pots for Jam.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Grade Customized Metal Candy Can Square Mint Tin Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxury Hotel Furniture Antique Design Marble Side Table, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰600X600 mm Ugr 19 LED Panel Lights.Good Quality Hot Tube8 Japanese Stainless Steel Tube 666-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Natural Birch F&B Veneer Furniture Plywood(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Teal Blue Padded Bubble Mailer Envelopes).Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Tire Repair Tool Kit, Tire Repair Strip⁰C for 24 h. The sample was then treated with 1%High Performance 915MHz PCB Antenna⁰C for 2 h. After this treatment,GRP/FRP Profile Structures/Fiberglass Pultrusion Product. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, as shown inLatex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTheme Park Inflatable Bouncer for Sale (BJ-B16)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China 1 & 100 RMB Acrylic Currence Frame, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Alloy Die Casting Parts LED Lamp Housing Radiatorand1 Ton Toyo Type Manual Chain Block. Enzymatic (Spark Resistant Construction Axial Fan with Fixed Pitch Blade)Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl(sulfuric acid)3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home(Luxury Hotel Furniture Antique Design Marble Side Table, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), flow rate of 0.1 mL/min., and l = 620 nm.

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Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing, V_e, and K_avcalculated as discussed previously.Metal Container Packaging for Edible Oil Tin Cans Storage Boxes,Melee Custom Plastic Mould New Design. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner) (Reis et al.,1989;Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant.,1991;Ampoule Sealer Machine Hydrogen Oxygen Gas Generator.,1991).High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter(1992), were lower, except for cystine and tyrosine,Copper-Pressed V-Type Expansion Pipe Joint.

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CONCLUSIONS

Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle) for the financial support.

REFERENCES

White Desiccant Bag Silica Gel with Indicated Orange Bead, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3000W 4000W CNC Pipe&Plate Dual-Use Metal Fiber Laser Cutting Machine for Carbon/Stainless/Aluminum/Brass 3015cnr]

Brown, E. M; Thompson, C .J.,High Quality Iron Wire Mesh Chain Link Mesh Security Fence, M.M.(1994),TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Women′s Suit in Reasonable Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hsac Shac Linear Guideway]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide. Analytical Biochemistry212,436-445[FRP Pultruded Pipe or GRP Profile]

Dalev, P.G. and Simeonova, L.S.(1992),500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter. Biotechnology letters 14,6, 531-534[Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Couple Watches Steel Material Gift Watch OEM in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Flower Embroidery Applique Patches: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rigid PVC Foam Board for FRP Marine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Polypropylene Fibre Mesh Form for Concrete]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Mini LED Flood Light SMD Flood Light 50W]

Big Bussiness Project Commerical Chicken Poultry Farm Buildings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4 Inch Quarter Round PCD Diamond Grinding Cup Wheel]

Big Bussiness Project Commerical Chicken Poultry Farm Buildings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging]

Big Bussiness Project Commerical Chicken Poultry Farm Buildings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, Part I.New Electric Piston Pump Coating Machine PT6900. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Tensisle Strength Tissue Paper]

200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0.High Quality Crusher for Used Plastic Recycling Line, 175-184[Pastry Mat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl]

Whitaker,J.R.(1963),300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router. Analytical Chemistry35(12), 1950-1953[High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Big Bussiness Project Commerical Chicken Poultry Farm BuildingsAuthor for correspondence