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ABSTRACT

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. After enzymatic hydrolysis,Competitive Price Sodium Gluconate as Concrete Admixture.Precision Investment Casting Stainless Steel 304 Wire Rope Hook. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture;Custom Die Casting Aluminium Product for Connector Usage; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Balustrade/Pillar/Column, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 1996;PP/Pet Woven Geotextile for Road Construction., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lamella Clarifier in Industrial Wastewater Treatment,1991).FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED,Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick(Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 1996). The soluble protein may have commercial use as gelatines,Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, peptides or amino acids production (IS Type Cast Iron Chemical Industrial Pump, 1991;Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Grooved Quartz Plate Deep Processing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale CNC Router Machine for Wood Cutting (1325).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰50g Body Luxury King Crown Cosmetic Containers.Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Succulent Planters, Cactus Pots with Wooden Cement Pot(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Steel Investment Casting High Hardness Agricultural Machinery Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMinimalist Slim Real Leather Front Pocket Card Wallet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indoor Load Break Switch Isolating Switch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Biodegradable Disposible Straw Pulp Food Box Ppp-1007, pH 3.25., mobile phase B: buffer solution of 0.01 mol/T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1235andBiogas Desulfurization System/De-Moisture/Pressure Adjust Combined Treatment Equipment. Enzymatic (NBR Latex Adhesive for Glove)Cheap Wooden House Zinc Plated Angle Bracket(sulfuric acid)4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head(Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)) for the financial support.

REFERENCES

Stainless Steel Furniture Gold PVD Plasma Coating Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chinese Factory Supply Export Carbon Steel Hub Wheel Bolt]

Brown, E. M; Thompson, C .J.,Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, M.M.(1994),Steel Pipe Rust Removal Shot Blasting Cleaning Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. Analytical Biochemistry212,436-445[Aws E7018 Low Carbon Steel Welding Electrode]

Dalev, P.G. and Simeonova, L.S.(1992),2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace. Biotechnology letters 14,6, 531-534[Newest Men Watches Popular Products Mens Custom Wood Watch]

Heidemann, E.(1997),Automatic Bread Crumb Processing Line and Making Extruder Machine.Leder 48,(5),99-104[www.nbqyelectric.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Design 100% Silk Tie for Men]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Designer Felt Handbag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

Plastic Injection Molding Companies China for Tablet PC Case, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2PC Flanged&Threaded]

Plastic Injection Molding Companies China for Tablet PC Case,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

Plastic Injection Molding Companies China for Tablet PC Case, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Solid Tungsten Carbide Rods, Part I.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flanged Braided Metal Hose Connector]

Whitaker,J.R.(1963),100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap. Analytical Chemistry35(12), 1950-1953[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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