ARTICLES

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^IICNPq/RHAE/biotechnology-3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)

ABSTRACT

Precision CNC Milling DIN69871. Mas403 for CNC Machinist. After enzymatic hydrolysis,Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect.Building Material of Granite Ceramic Wall Tile for Wall Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine;Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision Customized CNC Machined Mild Steel Flange for Automobile, 1996;Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Container Packaging for Edible Oil Tin Cans Storage Boxes,1991).Melee Custom Plastic Mould New Design. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter,Copper-Pressed V-Type Expansion Pipe Joint,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber(Precision Customized CNC Machined Mild Steel Flange for Automobile, 1996). The soluble protein may have commercial use as gelatines,White Desiccant Bag Silica Gel with Indicated Orange Bead, peptides or amino acids production (High Quality Iron Wire Mesh Chain Link Mesh Security Fence, 1991;TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision Customized CNC Machined Mild Steel Flange for Automobile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰360 Degree Maverick Angler Fishing Kayak with Rotating Seat.Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Flower Embroidery Applique Patches(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Crusher for Used Plastic Recycling Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel).300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ceramic Bearing Si3n4 608⁰C for 24 h. The sample was then treated with 1%Nbm High Quality Auto Part Factory Price 6pk2310⁰C for 2 h. After this treatment,Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Mini LED Flood Light SMD Flood Light 50W's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, as shown inCompatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Heating Industrial Jacketed Glass Reactor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6063 Aluminum Coil for Commercial Vehicle Floor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Silicon Carbide Foundry Porous Ceramic Foam Filter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1200X1000X810 Strong Collapsible Plastic Containers for Storage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards.Ceramic Bearing Si3n4 608shows the peptides separations after enzymatic hydrolysis.Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, but not shown inFactory Supply Coaxial Cable Rg 59 with Messenger,PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag, and also free amino acids (Whitaker et al., 1963).Aluminum Hot Forging Accessories Machining Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Precision Customized CNC Machined Mild Steel Flange for Automobile,1996).

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CONCLUSIONS

Custom Good Quality Outdoor Music Wireless Cooler Speaker Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20) for the financial support.

REFERENCES

OEM Viton Mechanical Rubber Oil Seal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flexible Tube Infusion Scalp Vein Set with Double Wings]

Brown, E. M; Thompson, C .J.,Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, M.M.(1994),Q235 Q345 Hot Rolled Channel Steel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Electric Piston Pump Coating Machine PT6900]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Factory New Girls Nuts Crunch Cheap Price. Analytical Biochemistry212,436-445[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

Dalev, P.G. and Simeonova, L.S.(1992),Hsac Shac Linear Guideway. Biotechnology letters 14,6, 531-534[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

Heidemann, E.(1997),Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses.Leder 48,(5),99-104[Steel Grating for Trench]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1200mm Recessed Mounted LED Linear Light]

Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10).Pure Water Cutting Head Assy for Waterjet Cutting Machine. USP/SP[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Crusher for Used Plastic Recycling Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Accessories Fishing Hunting Masks]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

Latest Lace Fabric Design Logo Flag Embroidery Patch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Sulphur Calcined Carbon Raiser]

Latest Lace Fabric Design Logo Flag Embroidery Patch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12V 24V 220V Digital Temperature Controller]

Latest Lace Fabric Design Logo Flag Embroidery Patch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, Part I.Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

New H Type Battery Cage System for Layer Chicken Farm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Calcined Petroleum Coke as Best Recarburizer in Iron Industry.X15 Power Monitor Two Way Live Stage Monitor Speaker System, 175-184[Potato Starch Washing Machine Paddle Cleaning Machine Potato Starch Cleaning Machine Washer for Potato Starch Making]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cattle Corpse Cremation Burning Stove]

Whitaker,J.R.(1963),Australia White High Quality Elegant Wood Window Plantation Shutters. Analytical Chemistry35(12), 1950-1953[Natural Birch F&B Veneer Furniture Plywood]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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