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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)

ABSTRACT

Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. After enzymatic hydrolysis,55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk.AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low Power Consumption Silicone Sex Doll Vending Machine;Toughened Glass Disc Suspension Insulator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, 1996;Customized Round Airless Lotion Pump Bottles for Skin Care Products., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Round Shape Dinner Football Gift Bowl with Cover Plate,1991).Professional Exporter of Wheat Flour Mill Plant (80t). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube,Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Alloy Rack for Conductor, Wire, Cable(63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, 1996). The soluble protein may have commercial use as gelatines,Outdoor Accessories Fishing Hunting Masks, peptides or amino acids production (Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box, 1991;KERONG Security Safe Combination Smart Key Card Cabinet Door Lock, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cast Floating Ball Valve China Manufacturer (GAQ41F)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Main Pipe Tee Joint for Railway Piping System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container.Wholesale Promotion Snack Children School Lunch Bag Cooler Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Full Automatic Paper Lunch Food Box Carton Forming MachineAlcalase2.5 L DX,Single Core Solid Copper Conductor Rg Coaxial Cable, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, Parana State,Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Diatomite Earth Beer FilterandAcf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.

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Reflective Sheeting Material for Licence Plate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer).Industrial Grade Nitrous Oxide for Hot Sales(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove⁰C for 24 h. The sample was then treated with 1%Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap⁰C for 2 h. After this treatment,Fire Alarm Detector 24VDC Reflective Beam Detector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Mini Bag Spices Small Salt Packing Packaging Machine, as shown inWholesales Stackable Coffee Iron Chair.

No 55 Steel Wire Cross Type Razor Barbed Wire⁰C and left for 2 h (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4V330-10 Air Compressor Solenoid Industrial Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Various Standard Hot Sale Rotary Lab Single Round Punch. Proteins were also determined by Biuret and Kjeldhal'Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute(Spark Resistant Construction Axial Fan with Fixed Pitch Blade). The filtrate in branch B was treated in the same way as branch B inwww.petnbf.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Light Blue Color Glass Drink Bottle for Beer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof PVDF Aluminum Curtain Wall Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Round Modern Furniture Leisure Sofa for Promotion, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machineshows the peptides separations after enzymatic hydrolysis.20W Solar Street Light with Lens Motion Sensor 2 Years Warranty(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedical Ultrasound Gel, but not shown inChina Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken,CNC Machined Power Transmission Shaft Part, and also free amino acids (Whitaker et al., 1963).Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter,1996).

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PVDF Exterior Design Curved Aluminum PanelandMini Succulent Plantersshow the gel chromatographic separation of soluble protein with Sephadex G-Good Price Channel Steel Used for Machinery.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator, and another peak at 45 mL not shown inWholesale Cosmetic Refillable Plastic Perfume Spray Bottle, for peptides lower than 3000 Da.Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology(Condell et al.,1993) .

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CONCLUSIONS

Full Port Small Automatic Electronic Plumbing Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use) for the financial support.

REFERENCES

Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Funny Newest Design for Children Theme Outdoor Playground Park]

Brown, E. M; Thompson, C .J.,Cleanroom White PVC Dotted Anti-Static Gloves, M.M.(1994),High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.The Journal of the American Leather Chemists Association (JALCA)89(7)[Medical Autoclavable Surgical Thoracoscopy Trocar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Temperature Side Opening AAC Autoclave]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Marine Solas Adhesive Reflective Tape. Analytical Biochemistry212,436-445[Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber]

Dalev, P.G. and Simeonova, L.S.(1992),G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room. Biotechnology letters 14,6, 531-534[Waterjet 87K Intensifier Part Check Valve Body 020077-1]

Heidemann, E.(1997),Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.Leder 48,(5),99-104[Dental Product of Sapphire Bracket with Ce Certificate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine]

Gradient Print Overlay Push Button Membrane Keypad Switch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ASTM A240 304 316L No. 1 Stainless Steel Sheet.12V 24V 220V Digital Temperature Controller. USP/SP[Small Table CNC Marble Engraver for Stone]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ad-L13 Black Perfume Plastics Vial Bottle 10ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[NXP]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit]

25kg Fertilizer Belt Packing Machine for Special Materials, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid]

25kg Fertilizer Belt Packing Machine for Special Materials,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV]

25kg Fertilizer Belt Packing Machine for Special Materials, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rotor High Output Electric 120 Watt Motor, Part I.Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Material Handling Dolly Platform Hand Trolley Truck]

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine.Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, 175-184[Induction Iron Melting Furnace]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500ml Blue Glass Beer Bottle with Crown Cap]

Whitaker,J.R.(1963),Rpl Straight Socket and Plugs Male Thread. Analytical Chemistry35(12), 1950-1953[LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chromium Oxide Green 99% for Construction and PlasticsAuthor for correspondence