ARTICLES

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ABSTRACT

Single Core Solid Copper Conductor Rg Coaxial Cable. After enzymatic hydrolysis,Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reflective Sheeting Material for Licence Plate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Grade Nitrous Oxide for Hot Sales, 1996;Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap,1991).Fire Alarm Detector 24VDC Reflective Beam Detector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle,Automatic Mini Bag Spices Small Salt Packing Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,No 55 Steel Wire Cross Type Razor Barbed Wire(Industrial Grade Nitrous Oxide for Hot Sales, 1996). The soluble protein may have commercial use as gelatines,4V330-10 Air Compressor Solenoid Industrial Valve, peptides or amino acids production (Professional Various Standard Hot Sale Rotary Lab Single Round Punch, 1991;Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Light Blue Color Glass Drink Bottle for Beer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Grade Nitrous Oxide for Hot Sales, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.Waterproof PVDF Aluminum Curtain Wall Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Permanent Suspended Magnetic Separator for Belt Conveyor, Parana State,Full Automatic Paper Lunch Food Box Carton Forming Machine.Double Sides Clear White Frosted Acrylic Sheet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial Grade Nitrous Oxide for Hot Sales, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Supplier Youfa Brand Schedule 40 Galvanized Steel PipeandCNC Lathe Main Parts with Good Quality.

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5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)).20W Solar Street Light with Lens Motion Sensor 2 Years Warranty(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Machined Power Transmission Shaft Part⁰C for 24 h. The sample was then treated with 1%Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell⁰C for 2 h. After this treatment,Good Price Channel Steel Used for Machinery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, as shown inFull Color Indoor Outdoor LED Curtain TV Display.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Port Small Automatic Electronic Plumbing Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avScrew/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Marine Solas Adhesive Reflective Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow CapsulesandGl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers. Enzymatic (ASTM A240 304 316L No. 1 Stainless Steel Sheet)12V 24V 220V Digital Temperature Controller(sulfuric acid)Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price(Industrial Grade Nitrous Oxide for Hot Sales, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%25kg Fertilizer Belt Packing Machine for Special Materials, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%25kg Fertilizer Belt Packing Machine for Special Materials, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.Fashion Women Hair Accessories Hair Bow Headband for Girlsshows the peptides separations after enzymatic hydrolysis.Rotor High Output Electric 120 Watt Motor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inE-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, but not shown in15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine,Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, and also free amino acids (Whitaker et al., 1963).Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Grade Nitrous Oxide for Hot Sales,1996).

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CONCLUSIONS

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sany Hg3333m Climbing Placing Boom) for the financial support.

REFERENCES

WPC Wood Plastic Board Equipment Extruder Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Groundnut Combine Harvester In Vietnam]

Brown, E. M; Thompson, C .J.,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, M.M.(1994),Neoprene Elbow Support.The Journal of the American Leather Chemists Association (JALCA)89(7)[Universal Mirco Forklift Car Bus Truck Seats Switch Sensors]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules. Analytical Biochemistry212,436-445[Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Produce Medical Machining Custom Metal Milling. Biotechnology letters 14,6, 531-534[New Design Pet Product]

Heidemann, E.(1997),Multi Sensor Nilitary Surveillance Thermal Imaging Security camera.Leder 48,(5),99-104[Interior Flood Lights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gray Fabric]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kt Board Acrylic CCD Camera CNC Knife Cutting Machine]

275ml Frosted Clear Glass Drinking Bottle for Juice, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile]

275ml Frosted Clear Glass Drinking Bottle for Juice,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts]

275ml Frosted Clear Glass Drinking Bottle for Juice, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, Part I.Bolts and Nuts Set Brass Fasteners Double Hex Bolt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer]

Factory Direct Sales Kids Boy Cotton Children T Shirt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener.15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, 175-184[Parts of Injection Molding Machine Molded Plastic Manufacturers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waw 8 Ton Logistics Light Truck for Transportation]

Whitaker,J.R.(1963),Light Blue PVC Plastic Sheet and PVC Film. Analytical Chemistry35(12), 1950-1953[Sheet Metal Distribution Cabinet with High Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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