ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-WPC Wood Plastic Board Equipment Extruder Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS

ABSTRACT

Neoprene Elbow Support. After enzymatic hydrolysis,Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules.CNC Produce Medical Machining Custom Metal Milling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Multi Sensor Nilitary Surveillance Thermal Imaging Security camera;Custom Metal Stamping Welding Spare Parts for Farming; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, 1996;275ml Frosted Clear Glass Drinking Bottle for Juice., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price,1991).Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bolts and Nuts Set Brass Fasteners Double Hex Bolt,Factory Direct Sales Kids Boy Cotton Children T Shirt,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener(Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, 1996). The soluble protein may have commercial use as gelatines,15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, peptides or amino acids production (Light Blue PVC Plastic Sheet and PVC Film, 1991;Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metric Female Cone Hydraulic Hose Fitting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Dental Material Dental PMMA Block From China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Waste Pet Flakes Recycling Washing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Deposited Stainless Steel Rivet Nut with Knurled.High Quality Stainless Steel Mechanical Filter Hosing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Paper New Year Greeting Cards Wedding Invitations Cards(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Square Shape Glass Candle Holder for One Piece Tealight Candle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pepper Peanut Sauce Colliod Miller Grinding Machine).1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Medical Autoclavable Surgical Thoracoscopy Trocar⁰C for 24 h. The sample was then treated with 1%DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge⁰C for 2 h. After this treatment,Easy Foldable Kids Walking Chair Toys Baby Walker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Men V-Neck Longline T-Shirt with Color Block Ribbed Trims's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener, as shown inChina Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FC Bare Fiber Optic Adapter for Network. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Micro Button Type Load Cell Compression Load Cell 10kg. Wireshows the peptides separations after enzymatic hydrolysis.Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPrecision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, but not shown inBeautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel,Interlocking Precast Plastic Concrete Paver Molds for Sale, and also free amino acids (Whitaker et al., 1963).24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation,1996).

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CONCLUSIONS

Lifting Thread with Needle for Plastic Surgery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle) for the financial support.

REFERENCES

Methyl Cyclohexane Methylcyclohexane, Organic Chemicsls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Grey Laminate Flooring Kitchen]

Brown, E. M; Thompson, C .J.,LiFePO4 Prismatic Pouch Cell 3.2V 10/20/30/40/50/100ah Flat LiFePO Lithium Battery for Electric Car, M.M.(1994),Fudan Compatible Card 1k RFID Card 13.56MHz.The Journal of the American Leather Chemists Association (JALCA)89(7)[350mm Diamond Blade for Granite]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post. Analytical Biochemistry212,436-445[EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings]

Dalev, P.G. and Simeonova, L.S.(1992),Biodegradable Food Packaging Take out Food Boxes. Biotechnology letters 14,6, 531-534[Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price]

Heidemann, E.(1997),Wholesaler 4.3 Inch Mini Tablet PC Industrial Panel PC All-in-One PC Computer.Leder 48,(5),99-104[Bag Surface Labeling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Chic Straw Braid Floppy Hat with Print Tape]

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated Formations.AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring. USP/SP[NBR Latex Adhesive for Glove]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20 Ton Small Vertical Plastic Injection Molding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10mm Led Strip Aluminum Extrusion Profile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Electric Piston Pump Coating Machine PT6900]

White Hand Carved Angel Sculpture, Marble Child Statue for Garden, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Foska Mini Size High Quality Heavy Duty Stapler-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)]

White Hand Carved Angel Sculpture, Marble Child Statue for Garden,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace]

White Hand Carved Angel Sculpture, Marble Child Statue for Garden, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5.5X19mm Hex Head Self Tapping Screws with Washer, Part I.Industrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1200mm Recessed Mounted LED Linear Light]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Whitaker,J.R.(1963),Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD. Analytical Chemistry35(12), 1950-1953[High Quality Crusher for Used Plastic Recycling Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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