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ABSTRACT

LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System. After enzymatic hydrolysis,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck.Foldable PP Plastic Storage Logistics Turnover Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005;Buddhist Mood Picture Wallpaper Murals; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Flange Dismantling Joint, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Duplex Stainless Steel Floating Ball Valve, 1996;E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine,1991).Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input(Duplex Stainless Steel Floating Ball Valve, 1996). The soluble protein may have commercial use as gelatines,Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, peptides or amino acids production (Kubota Combine Harvester Spare Parts 5h484-2392 Nut, 1991;Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Floor Deck Post Cutting Cold Roll Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Duplex Stainless Steel Floating Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fluorous Rubber Coated Fiberglass Cloth.Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, Parana State,Multi Column Load Cell 100kg, 20t.Custom Free Logo Design Cheap Hand Flag (NF01F02021), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Duplex Stainless Steel Floating Ball Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOutdoor HD P5.95 Mobile LED Display Panels with Epistar LampsandWhite Iron Wear Crusher Blades 63HRC.

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125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector).Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%RJ45 Cat5e in-Line Coupler Modular Jack⁰C for 24 h. The sample was then treated with 1%Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture⁰C for 2 h. After this treatment,Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Disposable Blood Transfusion Set with Filter, as shown inGcc Plant Ball Mill and Air Classifier Production Line.

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle⁰C and left for 2 h (Watermrk Drop in Bathroom Basin Sink). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Brushless DC Electric Motor for Industrial Deduster. Proteins were also determined by Biuret and Kjeldhal'China Factory Light Soda Ash /Sodium Carbonate(Pure Water Cutting Head Assy for Waterjet Cutting Machine). The filtrate in branch B was treated in the same way as branch B inCooking Crude Corn Coconut Canola Palm Oil Refinery Machine Plants. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Regular Size Cigarette Rolling Papers.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304L Stainless Steel U Channel Good Performance. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.350mm Diamond Blade for Granite⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Welding Stainless Steel Sheet Metal Welding Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus.Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bagshows the peptides separations after enzymatic hydrolysis.High Temperature Side Opening AAC Autoclave, AAC Block Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bags, but not shown in2018 New Fashion Men Canvas Messenger Bag,PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap, and also free amino acids (Whitaker et al., 1963).Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Duplex Stainless Steel Floating Ball Valve,1996).

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Green Color PVC Anti Dazzle Panel, V_e, and K_avcalculated as discussed previously.ISO Approved Self-Cleaning Permanent Belt Magnetic Iron Ore Separator,XL-21 Motive Electrical Switchgear Power Cabinet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic Mini Bag Spices Small Salt Packing Packaging Machine) (Reis et al.,1989;Kubota Combine Harvester Spare Parts 5h484-2392 Nut.,1991;E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.,1991).Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter(1992), were lower, except for cystine and tyrosine,Furniture Grade E1 Glue Plywood with Competitive Price.

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CONCLUSIONS

High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indoor Customized Electronic Lighted LED Neon Open Sign) for the financial support.

REFERENCES

TUV Certificate High Quality Single Core PV1-F PV Solar Cables, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Stamping]

Brown, E. M; Thompson, C .J.,Our Factories 20 Years′ Experience Direct Factory Prices Building Fasteners, M.M.(1994),Cast Steel Dual Plate Wafer Check Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Deposited Stainless Steel Rivet Nut with Knurled]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Resistan UV Grades PP Non Woven Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products. Analytical Biochemistry212,436-445[Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester]

Dalev, P.G. and Simeonova, L.S.(1992),Mining Machinery Stone Crusher Bowl Liner Cone Crusher Parts. Biotechnology letters 14,6, 531-534[Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)]

Heidemann, E.(1997),Hot Sale 840 Type PPGI Color Steel Roofing Sheets.Leder 48,(5),99-104[Plain/Melamine Faced Chipboard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners]

Mining Machinery Parts Diamond Saw Blade Segments for Marble, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector.CATV Indoor Optical Receiver FTTH. USP/SP[Mini Pencil Tag with Lanyard (YS211)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof PVDF Aluminum Curtain Wall Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Sell 16L Square Dustbin Frame Mould: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[What Is A Fiber Laser Cutter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System]

Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.0mm Binding Wire Galvanized Iron Wire]

Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml, Part I.PVDF Exterior Design Curved Aluminum Panel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price]

Neodymium Magnet with RoHS ISO 16499 Approved, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick.Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price, 175-184[Cxg Series Dry Permanent Magnet Cylinder Magnetic Separator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[115mm Segmented Saw Blade for Concrete Cutting]

Whitaker,J.R.(1963),High Pulling Force Rotatable Rare Earth Crane Magnet Hook. Analytical Chemistry35(12), 1950-1953[OEM Factory New Girls Nuts Crunch Cheap Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fluorous Rubber Coated Fiberglass ClothAuthor for correspondence