ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-4V330-10 Air Compressor Solenoid Industrial Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professional Various Standard Hot Sale Rotary Lab Single Round Punch

ABSTRACT

Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute. After enzymatic hydrolysis,Light Blue Color Glass Drink Bottle for Beer.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor;Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof PVDF Aluminum Curtain Wall Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, 1996;Round Modern Furniture Leisure Sofa for Promotion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket,1991).Sany Hg3333m Climbing Placing Boom. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Packaging Tray Vacuum Forming Machine,Permanent Suspended Magnetic Separator for Belt Conveyor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Sides Clear White Frosted Acrylic Sheet(Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, 1996). The soluble protein may have commercial use as gelatines,5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, peptides or amino acids production (20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, 1991;CNC Machined Power Transmission Shaft Part, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Channel Steel Used for Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Direct Sale Patent up to Down Rubber Tile Curing Press(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, Parana State,Professional Various Standard Hot Sale Rotary Lab Single Round Punch.Cleanroom White PVC Dotted Anti-Static Gloves, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMagnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20andNew Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine.

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High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe).Marine Solas Adhesive Reflective Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room⁰C for 24 h. The sample was then treated with 1%Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers⁰C for 2 h. After this treatment,ASTM A240 304 316L No. 1 Stainless Steel Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V 24V 220V Digital Temperature Controller's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, as shown inDesign Luxury Moon Cake Box with Hot Stamp Logo.

25kg Fertilizer Belt Packing Machine for Special Materials⁰C and left for 2 h (Minimalist Slim Real Leather Front Pocket Card Wallet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rotor High Output Electric 120 Watt Motor. Proteins were also determined by Biuret and Kjeldhal'Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby(China 1 & 100 RMB Acrylic Currence Frame). The filtrate in branch B was treated in the same way as branch B inPet Food Bevel Double Bowl Bamboo Ceramic Double Bowl Cat Dog Bowl Pet Supplies Cat Bowl Pet Bowl. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Metal Stamping Stainless Steel Parts with CNC Machining, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ss Wafer Type Check Valve Dn32 Dn40 Dn50, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Air Purification Activated Aluminaand2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. Enzymatic (Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape)8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera(sulfuric acid)WPC Wood Plastic Board Equipment Extruder Machine(Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, flow rate of 0.1 mL/min., and l = 620 nm.

Neoprene Elbow Support.100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Priceshows the peptides separations after enzymatic hydrolysis.Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSany Hg3333m Climbing Placing Boom, but not shown inTeal Blue Padded Bubble Mailer Envelopes,CNC Produce Medical Machining Custom Metal Milling, and also free amino acids (Whitaker et al., 1963).Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas,1996).

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275ml Frosted Clear Glass Drinking Bottle for Juice, V_e, and K_avcalculated as discussed previously.EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price,Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Metal Stamping Welding Spare Parts for Farming) (Reis et al.,1989;20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.,1991;Round Modern Furniture Leisure Sofa for Promotion.,1991).Bolts and Nuts Set Brass Fasteners Double Hex Bolt(1992), were lower, except for cystine and tyrosine,Factory Direct Sales Kids Boy Cotton Children T Shirt.

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CONCLUSIONS

Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)) for the financial support.

REFERENCES

15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Elbow]

Brown, E. M; Thompson, C .J.,Light Blue PVC Plastic Sheet and PVC Film, M.M.(1994),Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hydraulic Press Corrugated Cold Roll Automatically Forming Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D Creative Design Metal Medal Promotion Gift of Sport]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metric Female Cone Hydraulic Hose Fitting. Analytical Biochemistry212,436-445[Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Dental Material Dental PMMA Block From China. Biotechnology letters 14,6, 531-534[High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale]

Heidemann, E.(1997),Plastic Waste Pet Flakes Recycling Washing Machine.Leder 48,(5),99-104[Kme Knife Sharpener]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner]

No 55 Steel Wire Cross Type Razor Barbed Wire, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Deposited Stainless Steel Rivet Nut with Knurled.High Quality Stainless Steel Mechanical Filter Hosing. USP/SP[2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Bulb Light UFO Light Recruitment Distributor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Paper New Year Greeting Cards Wedding Invitations Cards: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Women′s Suit in Reasonable Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Decorative Pillows]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hsac Shac Linear Guideway]

Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),BLE5.0 Ttl to Bluetooth Module with at Command-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FRP Pultruded Pipe or GRP Profile]

Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts]

Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, Part I.Professional Customization Screen Printing Machine for Porous Ceramic. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping]

Fea040b New Arrival Airplane Seat Belt Extender, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving.Square Shape Glass Candle Holder for One Piece Tealight Candle, 175-184[400W High Power Factory Directly IP65 Reflector LED Floodlight]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Outdoor Simple Gazebo Beer Festival Marquee Tent]

Whitaker,J.R.(1963),1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box. Analytical Chemistry35(12), 1950-1953[High Quality Couple Watches Steel Material Gift Watch OEM in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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