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ABSTRACT

Shac Flange Linear Bearing. After enzymatic hydrolysis,China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price.Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Router for Engraving Cutting Carving Drilling Milling;Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, 1996;High Quality Hot Rolled Steel U Channel China Supplier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (M Type Metallic Yarn Lurex Yarn Embroidery Yarn,1991).Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck,500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable(Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, 1996). The soluble protein may have commercial use as gelatines,Anti-Pollution Modified Acrylic Solid Surface for Countertops, peptides or amino acids production (Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, 1991;Corrugated Carton Box Fully Automatic Folding Gluing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;800lb 1/2 A105 Thread Forged Steel Globe Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machining Metal Mechanical Engineering Parts.Low Sulphur Calcined Carbon Raiser-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Free Sample Natural Fragrant Bath SPA Salt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Copper-Pressed V-Type Expansion Pipe Joint).Mobile Hydraulic Concrete Placing Boom(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700⁰C for 24 h. The sample was then treated with 1%Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll⁰C for 2 h. After this treatment,Cr1000 Spray Nozzle Common Rail Injector Tester. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Cast Steel Flanged One Way Swing Check Valve Nrv's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black White Stone 925 Silver Cross Pendants Necklace Jewelry, as shown in16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive.

Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People⁰C and left for 2 h (Dark Red Teflon Encap Silicone O Ring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17. Proteins were also determined by Biuret and Kjeldhal'Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced(300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router). The filtrate in branch B was treated in the same way as branch B inSintering Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Garvenment Projector High Mast Stadium Lighting High Power Lamp Outdoor LED Flood Light with 400 Watts, 5700 Kelvin, 277 Volts, 60 Hz⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carbon Steel Stainless Steel Countersunk Head Hollow Bolt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wearshows the peptides separations after enzymatic hydrolysis.Stainless Steel Bend Pipe for Industrial Use(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, but not shown inFashion Style Lady Alloy Quartz Watch with Simple Dial,Ybz-E16f20 Vane Steering Hydraulic Pumps, and also free amino acids (Whitaker et al., 1963).Good Quality Snow White Pumpkin Seeds for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer,1996).

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Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, V_e, and K_avcalculated as discussed previously.Watermrk Drop in Bathroom Basin Sink,OEM ABS Industrial Plastic Sheet Injection Auto Part. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01)) (Reis et al.,1989;Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose.,1991;High Quality Hot Rolled Steel U Channel China Supplier.,1991).15L Big Cooling Cylinder High Production Hard Ice Cream Machine(1992), were lower, except for cystine and tyrosine,Flange Type Ball Float Water Tank Control Valve.

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CONCLUSIONS

925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Deep Hole Cylinder Boring Machine for Sale) for the financial support.

REFERENCES

Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[GSM Series Antenna Customized Connector Flat GSM Antenna]

Brown, E. M; Thompson, C .J.,Customized Outdoor Plastic Chrome Auto Logo Signs, M.M.(1994),Small Table CNC Marble Engraver for Stone.The Journal of the American Leather Chemists Association (JALCA)89(7)[Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6000 Series Anodizing Extrusion Aluminum Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Thymosin Beta 4 Acetate Tb-500 Acetate 77591-33-4. Analytical Biochemistry212,436-445[High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production]

Dalev, P.G. and Simeonova, L.S.(1992),China Soundproof Construction Foam PVC/WPC Board. Biotechnology letters 14,6, 531-534[Professional Manufacture Metal Medal Promotional Gift]

Heidemann, E.(1997),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.Leder 48,(5),99-104[Floor Machine Granite Floor Polisher]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gradient Print Overlay Push Button Membrane Keypad Switch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Digital Modulator With Low Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[AC4 Grade Home and Commercial Use Wooden HDF Laminate Flooring]

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket]

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Oak Veneer Laminated Plywood for Decoration]

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, Part I.55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women]

AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Power Consumption Silicone Sex Doll Vending Machine.Toughened Glass Disc Suspension Insulator, 175-184[Sticker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[12 Inch King Mattress Latex Foam Memory Foam Mattress]

Whitaker,J.R.(1963),Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller. Analytical Chemistry35(12), 1950-1953[Double Door Heated Holding Carts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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