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ABSTRACT

3-Pin Australia Power Cord Plug with SAA. After enzymatic hydrolysis,Sludge Oil Separation Centrifuge.8011 Aluminum Foil for Cap Used. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pepper Peanut Sauce Colliod Miller Grinding Machine;Gasoline Rototiller, Cultivator, Tiller Model Gtm-6; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electronic Foldable for Patient Treatment Ent Unit Chair, 1996;Plastic Pushbutton Ultrasonic Spin Welding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles,1991).Double Shaft Paper Cardboard Waste Plastic Shredder Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards,Desktop Single Wave Soldering Machine/Wave Soldering Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturers White Dinnerware Set Fast Food Tray Bento Box(Electronic Foldable for Patient Treatment Ent Unit Chair, 1996). The soluble protein may have commercial use as gelatines,Best Price Graphite Electrode HP 300mm UHP, peptides or amino acids production (Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, 1991;Wld8200 Auto Spray Paint Booth, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Animal Carcass Incinerator Hospital Garbage Incinerator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electronic Foldable for Patient Treatment Ent Unit Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Density Crust Foam Board/Celuka Board.Mango Pulp Juice Processing Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Laser Cut Sheet Metal Fiber Laser Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

130kw 7 Degrees Scroll Type Industrial Water Chiller, Parana State,LED Outdoor Solar Lawn Garden Lighting for Wall.Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Electronic Foldable for Patient Treatment Ent Unit Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Performance 915MHz PCB AntennaandHigh Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.

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Factory Wholesale Melamine Garment Shop Furniture Design for Store(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Quick Connect Camlock and Grooved Couplings Quick Fitting Type D).Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Comfortable Men′s Winter Leather Snow Boots⁰C for 24 h. The sample was then treated with 1%Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality⁰C for 2 h. After this treatment,Gcc Plant Ball Mill and Air Classifier Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Playground Plastic Slide with Swing and Basketry, as shown inWholesale Comfortable Men′s Winter Leather Snow Boots.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1.8ml Clear Sample Vials with Label and PP Caps. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Small Coil Compression Spring in High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Material Handling Dolly Platform Hand Trolley Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fashionable Natural Bamboo Wood Sunglasses for Men.New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutteshows the peptides separations after enzymatic hydrolysis.Solar Powered Outdoor Lighting LED Solar Wall Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, but not shown in41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45),Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, and also free amino acids (Whitaker et al., 1963).9~12 Inch Mechanical Backing Plate. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electronic Foldable for Patient Treatment Ent Unit Chair,1996).

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Ozone Generator Plant for Water Treatment, V_e, and K_avcalculated as discussed previously.En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator,Free Assembly, Walking Machine, Home Fitness, Treadmill. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System) (Reis et al.,1989;Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.,1991;Plastic Pushbutton Ultrasonic Spin Welding Machine.,1991).CMC Transparent Grade for Coating Production(1992), were lower, except for cystine and tyrosine,Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine.

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CONCLUSIONS

Cold-Spray Road Line Marking Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Sexy Crystal Self Adhesive Resuable Nipple Cover) for the financial support.

REFERENCES

Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best quality Antique Metal Table Lamp -]

Brown, E. M; Thompson, C .J.,Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel, M.M.(1994),Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes.The Journal of the American Leather Chemists Association (JALCA)89(7)[The Big Diameter Carbide Tipped Hole Saw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2). Analytical Biochemistry212,436-445[New Sexy Crystal Self Adhesive Resuable Nipple Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950. Biotechnology letters 14,6, 531-534[NPK+Te Fertilizer]

Heidemann, E.(1997),Rock Tunnel Boring Machine for Natural Gas Pipeline.Leder 48,(5),99-104[Decorative Organize Box]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cold-Spray Road Line Marking Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Singlemode Su Upc Fiber Optic Fast Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Double Acting Hydraulic Cylinder for Excavator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Motor Company Produce BLDC Fan Motor for Barn Vent Fan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gypsum Board False Ceiling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap CNC Spindle Peeling Lathe for Steel Bar]

Fashion High Quality Head Layer Genuine Leather Men′s Wallet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Teal Blue Padded Bubble Mailer Envelopes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste]

Fashion High Quality Head Layer Genuine Leather Men′s Wallet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Light Blue PVC Plastic Sheet and PVC Film]

Fashion High Quality Head Layer Genuine Leather Men′s Wallet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Ultrasound Gel, Part I.Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Foldable PP Plastic Storage Logistics Turnover Box]

Wholesale Blank Promotional Baseball Cap for Custom Logo Design, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gear Rack and Pinion for Construction Hoist, Construction Spare Parts.Power Plant Seawater Level Measurement Integrated Pressure Transducer, 175-184[595 Easy Installation Plastic Suspended Ceiling Design]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer]

Whitaker,J.R.(1963),High Quality Melamine Paper Manufacturer for Decoration. Analytical Chemistry35(12), 1950-1953[Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

En471 Reflective Fabric Tape Cintas Reflectantes De Tela PlateadaAuthor for correspondence