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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Delicate Handmade Round Natural Stone Chain Necklace, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, 1996;Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Embossed Aluminium/ Aluminum Household Foil 8011-O,1991).High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle,Cross Hex Flange Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings(Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, 1996). The soluble protein may have commercial use as gelatines,Full Color Indoor Outdoor LED Curtain TV Display, peptides or amino acids production (High Quality Plastic Injection Mold Parts Square Interlocks Maker, 1991;LED Outdoor Solar Lawn Garden Lighting for Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3-Pin Australia Power Cord Plug with SAA., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sludge Oil Separation Centrifuge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8011 Aluminum Foil for Cap Used. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pepper Peanut Sauce Colliod Miller Grinding Machine.Gasoline Rototiller, Cultivator, Tiller Model Gtm-6-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12.5mva Transformer 12500kVA 33kv 35kv Power Transformer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Manufacturers White Dinnerware Set Fast Food Tray Bento Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight).Best Price Graphite Electrode HP 300mm UHP(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1⁰C for 24 h. The sample was then treated with 1%Wld8200 Auto Spray Paint Booth⁰C for 2 h. After this treatment,Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Animal Carcass Incinerator Hospital Garbage Incinerator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, as shown inGaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Sexy Crystal Self Adhesive Resuable Nipple Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCandy Box Laser Marking Engraving Machine Scm2000 180W 250W(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Wholesale Melamine Garment Shop Furniture Design for Store, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles) for the financial support.

REFERENCES

ATV Spiked Chain Harrow for Farming, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lab Glassware Test Tube]

Brown, E. M; Thompson, C .J.,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60, M.M.(1994),Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody.The Journal of the American Leather Chemists Association (JALCA)89(7)[Motorcycle Tube Making Machine/Inner Tube Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashionable Natural Bamboo Wood Sunglasses for Men. Analytical Biochemistry212,436-445[High Speed Steel Mill Roll with High Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Solar Powered Outdoor Lighting LED Solar Wall Light. Biotechnology letters 14,6, 531-534[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

Heidemann, E.(1997),Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs.Leder 48,(5),99-104[Farm Automatic Chick Duck Battery Layer Cage Water Nipples Equipment Poultry Nipple Drinker for Broiler Chicken]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Price 4X6 Acrylic Magnetic Photo Frame]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Window System Thermal Break Strip (HK Stype35.3mm): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Cooler Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

LED Bulb Light UFO Light Recruitment Distributor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ozone Generator Plant for Water Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wld8200 Auto Spray Paint Booth]

LED Bulb Light UFO Light Recruitment Distributor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Biodegradable Disposible Straw Pulp Food Box Ppp-1007]

LED Bulb Light UFO Light Recruitment Distributor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator, Part I.Free Assembly, Walking Machine, Home Fitness, Treadmill. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[NPK 16X Hammer Spare Parts Racing Forged Piston]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder]

Whitaker,J.R.(1963),Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner. Analytical Chemistry35(12), 1950-1953[4V330-10 Air Compressor Solenoid Industrial Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

1 Ton Toyo Type Manual Chain BlockAuthor for correspondence