ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom

ABSTRACT

Free Shipping Wholesale Peruvian Human Hair Weave. After enzymatic hydrolysis,Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags.Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Polypropylene Strapping Band Production Line/PP Strap Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, 1996;12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500ml Blue Glass Beer Bottle with Crown Cap,1991).White Iron Wear Crusher Blades 63HRC. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System,Stone Carving Flower Pot for Garden Decoration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2018 New Fashion Men Canvas Messenger Bag(China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, 1996). The soluble protein may have commercial use as gelatines,High Purity Sodium Bicarbonate with White Powder Type, peptides or amino acids production (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, 1991;IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Carbide Rod H6 Tc Rods., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Mens Slim Wallet Custom Design Leather Wallet Men.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-St Fiber Optic Adaptor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Cute Animal Dinosaur Kids Baby Baseball Cap.Latest Fashion Best-Selling Marble Slab Cutting Saw Blades-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gcc Plant Ball Mill and Air Classifier Production Line).New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)⁰C for 24 h. The sample was then treated with 1%Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment⁰C for 2 h. After this treatment,Rht-060ws Soda Process Water Cooled Screw Type Water Chiller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, as shown inWatermrk Drop in Bathroom Basin Sink.

Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar⁰C and left for 2 h (Pure Water Cutting Head Assy for Waterjet Cutting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair. Proteins were also determined by Biuret and Kjeldhal'Flanged Braided Metal Hose Connector(500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter). The filtrate in branch B was treated in the same way as branch B inWallets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Timber Pressure Vessel for Wood Processing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1200mm Recessed Mounted LED Linear Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avThe Big Diameter Carbide Tipped Hole Saw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Men′s Large Dial Casual Quartz Watch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor Full Color LED Video Wall.2019 Hiking Sleeping Folding Beach Air Sofa Lazy Bagsshows the peptides separations after enzymatic hydrolysis.180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2018 New Fashion Men Canvas Messenger Bag, but not shown inCustom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card,Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, and also free amino acids (Whitaker et al., 1963).Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor,1996).

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CONCLUSIONS

Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Paper Automaitc Carton Box Cardboard Strapping Machine) for the financial support.

REFERENCES

Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wheat Flour Mill Grain Processing Equipment]

Brown, E. M; Thompson, C .J.,800mm Length 600n Load Tension Gas Spring with Metal Eyelet, M.M.(1994),Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003.The Journal of the American Leather Chemists Association (JALCA)89(7)[Resistan UV Grades PP Non Woven Fabric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fabric Coated PVC for Garment Woven Fabric 600d 100% Polyester]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. Analytical Biochemistry212,436-445[Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)]

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Heidemann, E.(1997),SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel.Leder 48,(5),99-104[Bakery Paper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini Pencil Tag with Lanyard (YS211)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Food Grade Customized Metal Candy Can Square Mint Tin Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Handmade Wedding Dress Diamond Rhinestone Applique Sash]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

600X600 mm Ugr 19 LED Panel Lights, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Quality Hot Tube8 Japanese Stainless Steel Tube 666-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1.0mm Binding Wire Galvanized Iron Wire]

600X600 mm Ugr 19 LED Panel Lights,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price]

600X600 mm Ugr 19 LED Panel Lights, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine, Part I.Round Plastic Hanging Basket (KD7014). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[115mm Segmented Saw Blade for Concrete Cutting]

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, 175-184[38mm Anchor Chain Swivel Group Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Factory New Girls Nuts Crunch Cheap Price]

Whitaker,J.R.(1963),New Design High Quality Alminum Sandwich Panel High Pressure Laminate. Analytical Chemistry35(12), 1950-1953[Fluorous Rubber Coated Fiberglass Cloth]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film MachineAuthor for correspondence