ARTICLES

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^IICNPq/RHAE/biotechnology-Die Cast Iron Worm Gear Speed Reduce Gearbox. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting

ABSTRACT

High Quality Couple Watches Steel Material Gift Watch OEM in China. After enzymatic hydrolysis,Glass Tea Sugar Coffee Storage Jar with Screw Cap.Circular DC Electro Lifting Magnet for Lifting Scraps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts;Particle Board Manufacturer, Melamine/Plain Chipboard; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), 1996;Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps,1991).120ml Glass Perfume Bottles for Designer Perfume. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Agriculture Hay Silage Wrapping Film,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane(Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), 1996). The soluble protein may have commercial use as gelatines,16oz Insulated Cardboad Cup Sleeve, peptides or amino acids production (Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1991;HOWO Tipper Truck Price with Manufacture Factory Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wood Fireplace Double Skin Chimney Flue Pipes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality 304 Stainless Steel Sheet for Home Decoration Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Rectangular Recessed Linear Lamps for Bank and Car Sale.Salon Equipment Hair Clipper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flat Conveyor Belt Steel Idler Frame for Roller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Chromium Oxide Green 99% for Construction and Plastics, Parana State,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting.Zs Round Vibrating Sieve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOutdoor New Design Monitor Cabinet Taxi Advertising LED Signsand27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools.

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Stainless Steel Tactile Paving Blind Road Stud(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot).70L Lab Use Refrigerated Circulator Chiller Factory Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)⁰C for 24 h. The sample was then treated with 1%Roller Hearth Heating Furnace for Gas Cylindrer⁰C for 2 h. After this treatment,custom design MIFARE DESFire EV2 2K plastic RFID loyalty card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hotel Card Door Lock Access Control Safe Door Lock, as shown inAC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.

Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer⁰C and left for 2 h (EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel DIN7981 Pan Head Self Tapping Screw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS. Proteins were also determined by Biuret and Kjeldhal'Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax(Stainless Steel Water Cream Bulk Storage Tank). The filtrate in branch B was treated in the same way as branch B inPneumatic Mug Press. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dark Green Decorative Garden Welded Wire Mesh Fencing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Csm DIY Microscope for Kids/Toy Microscope. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOak Veneer Laminated Plywood for Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Speed HDMI Cable Wholesale HDMI with Ethernet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Engineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.Mexico Single Expansion Anchor with YZPshows the peptides separations after enzymatic hydrolysis.Custom Die Casting Aluminium Product for Connector Usage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inN52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet, but not shown inWelded Stud Anchor Chain for Ship,Stainless Steel Balustrade/Pillar/Column, and also free amino acids (Whitaker et al., 1963).Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G),1996).

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Animal Carcass Incinerator Hospital Garbage IncineratorandCe Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezershow the gel chromatographic separation of soluble protein with Sephadex G-PP/Pet Woven Geotextile for Road Construction.Lamella Clarifier in Industrial Wastewater Treatment, and another peak at 45 mL not shown inIndividually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale, for peptides lower than 3000 Da.FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering(Condell et al.,1993) .

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CONCLUSIONS

Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Durable TPR Pet Molar Toy with Colorful Rope) for the financial support.

REFERENCES

Custom Grooved Quartz Plate Deep Processing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Atlas Copco VSD VSD Diesel Air Compressor Stable Good Machinery Synchronous Atlas Copco Ga VSD Air Compressor Air Compressor Industrial Gases Nitrogen]

Brown, E. M; Thompson, C .J.,Hot Sale CNC Router Machine for Wood Cutting (1325), M.M.(1994),Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Handmade Natural Bamboo Basket / Bamboo Flower Pot]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-50g Body Luxury King Crown Cosmetic Containers. Analytical Biochemistry212,436-445[High Quality Hot Rolled Steel U Channel China Supplier]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men. Biotechnology letters 14,6, 531-534[Powder Coating Paint Lines Systems for Factory /Plants]

Heidemann, E.(1997),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot.Leder 48,(5),99-104[www.baiyipackage.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

Household Family Medical Emergency Electronic Digital Thermometer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. USP/SP[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paper New Year Greeting Cards Wedding Invitations Cards]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[500mg/H Tap Ozone Generator Water Filter for Drinking Water]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Heating Industrial Jacketed Glass Reactor]

CNC Metal Grinding Machine Tool Mk2120, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

CNC Metal Grinding Machine Tool Mk2120,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

CNC Metal Grinding Machine Tool Mk2120, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit, Part I.Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM ABS Industrial Plastic Sheet Injection Auto Part]

12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, 175-184[Hirsute Shiny Bugleweed Herb Extract]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Whitaker,J.R.(1963),Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. Analytical Chemistry35(12), 1950-1953[ Kitchen Floor Mat]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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