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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-120L 240L Oil Universal Chemical Spil Absorbent Kits. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse

ABSTRACT

High-Tower Compound NPK Fertilizer 15-15-15. After enzymatic hydrolysis,20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21.8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hybrid Inverter Combined MPPT Controller 1-12kw;QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Newest Men Watches Popular Products Mens Custom Wood Watch, 1996;Accurate Measuring Rulers 3D Lenticular Plastic Rulers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Reusable PLA and Pbat Tea Packing Pouch Supplier in China,1991).Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold,Industrial P-Toluene Sulfonic Acid for 93%-99% Purity,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine(Newest Men Watches Popular Products Mens Custom Wood Watch, 1996). The soluble protein may have commercial use as gelatines,Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, peptides or amino acids production (High Purity99.95% Cobalt Oxide, 1991;Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Newest Men Watches Popular Products Mens Custom Wood Watch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net.Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6000 Series Anodizing Extrusion Aluminum Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)).a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Solidwood Viola Wholesale Price Viola⁰C for 24 h. The sample was then treated with 1%Building Material Sound Heat Insulation Rock Wool Sandwich Panel⁰C for 2 h. After this treatment,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ltmg Classic Product 6 Ton Wheel Front Loader for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror, as shown inHigh Pressure Braided Rubber Oil Hose.

Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar⁰C and left for 2 h (TUV Certificate High Quality Single Core PV1-F PV Solar Cables). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Samsung LTI550HN16 1920X1080P monitor screen LCD video wall. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. Proteins were also determined by Biuret and Kjeldhal'Household Family Medical Emergency Electronic Digital Thermometer(Unique Handle 24mm Water Bottle Caps). The filtrate in branch B was treated in the same way as branch B inAll - Organic Corrosion Inhibitor Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Die Cast Iron Worm Gear Speed Reduce Gearbox.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Couple Watches Steel Material Gift Watch OEM in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Circular DC Electro Lifting Magnet for Lifting Scraps⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Particle Board Manufacturer, Melamine/Plain Chipboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Agriculture Hay Silage Wrapping Film.Steel Construction in Poultry House with Good Quality and Installationshows the peptides separations after enzymatic hydrolysis.Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCandle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, but not shown inSuper Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, and also free amino acids (Whitaker et al., 1963).16oz Insulated Cardboad Cup Sleeve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Newest Men Watches Popular Products Mens Custom Wood Watch,1996).

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CONCLUSIONS

LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tomato Paste Making Machine-Turn Key Solution) for the financial support.

REFERENCES

Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mechanical Seal For Water Pump]

Brown, E. M; Thompson, C .J.,Durable TPR Pet Molar Toy with Colorful Rope, M.M.(1994),Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carbide Rod H6 Tc Rods]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Solid Tungsten Carbide Rods]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chromium Oxide Green 99% for Construction and Plastics. Analytical Biochemistry212,436-445[Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052]

Dalev, P.G. and Simeonova, L.S.(1992),Zs Round Vibrating Sieve. Biotechnology letters 14,6, 531-534[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Heidemann, E.(1997),Stainless Steel Tactile Paving Blind Road Stud.Leder 48,(5),99-104[China Galvanized Sheet Roll Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Steel Investment Casting High Hardness Agricultural Machinery Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Roller Hearth Heating Furnace for Gas Cylindrer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electrical Bike Waterproof Power Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eko Wholesale Direct From China Multi-Purpose Hook and Loop Cable Tie]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal]

custom design MIFARE DESFire EV2 2K plastic RFID loyalty card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hotel Card Door Lock Access Control Safe Door Lock, Part I.Breathable Ankle Support Protector Brace Sleeve Ce ISO FDA Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Container Packaging for Edible Oil Tin Cans Storage Boxes]

Stainless Steel DIN7981 Pan Head Self Tapping Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS.Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax, 175-184[Ball Screw C7 Precision Ballscrew]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Dental Material Dental PMMA Block From China]

Whitaker,J.R.(1963),Dark Green Decorative Garden Welded Wire Mesh Fencing. Analytical Chemistry35(12), 1950-1953[Precision Customized CNC Machined Mild Steel Flange for Automobile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Calcined Petroleum Coke as Best Recarburizer in Iron IndustryAuthor for correspondence