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ABSTRACT

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. After enzymatic hydrolysis,Copper-Pressed V-Type Expansion Pipe Joint.Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;White Desiccant Bag Silica Gel with Indicated Orange Bead;High Quality Iron Wire Mesh Chain Link Mesh Security Fence; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, 1996;500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera,1991).360 Degree Maverick Angler Fishing Kayak with Rotating Seat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera,Wholesale Flower Embroidery Applique Patches,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Big Bussiness Project Commerical Chicken Poultry Farm Buildings(5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, 1996). The soluble protein may have commercial use as gelatines,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, peptides or amino acids production (Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, 1991;New Electric Piston Pump Coating Machine PT6900, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Crusher for Used Plastic Recycling Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.Ceramic Bearing Si3n4 608-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nbm High Quality Auto Part Factory Price 6pk2310(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Manufacture Metal Medal Promotional Gift, Parana State,Melee Custom Plastic Mould New Design.Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)andActive Demand Plastic 65 Liter Laundry Basket Mould.

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Singlemode Su Upc Fiber Optic Fast Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope).Roller Skating Sneakers Shoes for Children (8083)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder⁰C for 24 h. The sample was then treated with 1%Electric Heating Industrial Jacketed Glass Reactor⁰C for 2 h. After this treatment,40X-1600X Biological Microscope Xsz-107V LED Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6063 Aluminum Coil for Commercial Vehicle Floor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silicon Carbide Foundry Porous Ceramic Foam Filter, as shown inFlying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe.

1200X1000X810 Strong Collapsible Plastic Containers for Storage⁰C and left for 2 h (SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel Construction in Poultry House with Good Quality and Installation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White. Proteins were also determined by Biuret and Kjeldhal'10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle(High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn). The filtrate in branch B was treated in the same way as branch B inBeautiful Wall Water Fountain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stone Cracking Rock Cutting Cement.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Tk4100 125kHz RFID Printing Plastic Student ID Cards(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Hot Forging Accessories Machining Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Building Wall Machine for Hot Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Plastic Injection Mold Parts Square Interlocks MakerandNew Arrival Amazon Hot Style Printed Slip Dress for Women Summer. Enzymatic (Metal Cuting CNC Plasma Cutting Machine (DW1325))Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant(sulfuric acid)Expanded Metal Infill Panel Metal Mesh Custom Made(5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%DC Motor for Massagers, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%DC Motor for Massagers, flow rate of 0.1 mL/min., and l = 620 nm.

High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage.High Quality Suitcase Frame or Profile Bending Machine with Ce Aprrovedshows the peptides separations after enzymatic hydrolysis.Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSeasoned Cast Iron South Africa Three Legged Potjie Pot, but not shown inFood Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine,Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, and also free amino acids (Whitaker et al., 1963).OEM Viton Mechanical Rubber Oil Seal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide,1996).

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Hsac Shac Linear Guideway, V_e, and K_avcalculated as discussed previously.Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses,Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Belt Conveyor Systmem for Fertilizer Production Line) (Reis et al.,1989;Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20.,1991;500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter.,1991).Pure Water Cutting Head Assy for Waterjet Cutting Machine(1992), were lower, except for cystine and tyrosine,Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat.

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CONCLUSIONS

Latest Lace Fabric Design Logo Flag Embroidery Patch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead) for the financial support.

REFERENCES

China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5L Supercritical CO2 Extraction Machine for Cbd Oil]

Brown, E. M; Thompson, C .J.,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, M.M.(1994),Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05.The Journal of the American Leather Chemists Association (JALCA)89(7)[Round Shape Dinner Football Gift Bowl with Cover Plate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New H Type Battery Cage System for Layer Chicken Farm. Analytical Biochemistry212,436-445[Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Calcined Petroleum Coke as Best Recarburizer in Iron Industry. Biotechnology letters 14,6, 531-534[Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine]

Heidemann, E.(1997),X15 Power Monitor Two Way Live Stage Monitor Speaker System.Leder 48,(5),99-104[12v Dc Self-Priming Water Pump Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rpl Straight Socket and Plugs Male Thread]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flat Conveyor Belt Steel Idler Frame for Roller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bwg9 Galvanized Iron Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box]

Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Custom Sheet Metal Fabrication-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet]

Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aws E7018 Low Carbon Steel Welding Electrode]

Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, Part I.Powder Coating Paint Lines Systems for Factory /Plants. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Newest Men Watches Popular Products Mens Custom Wood Watch]

USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mini Pencil Tag with Lanyard (YS211).100% Latex Free Endotracheal Tube with PVC Material, 175-184[Plastic Injection Mold Manufacture for The Coffee Machine Shell]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

Whitaker,J.R.(1963),Neodymium Magnet Pot Assembly with Strong Weight. Analytical Chemistry35(12), 1950-1953[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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