ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Plastic Material Handling Dolly Platform Hand Trolley Truck. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump

ABSTRACT

Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes. After enzymatic hydrolysis,99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5.ATV Spiked Chain Harrow for Farming. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashionable Natural Bamboo Wood Sunglasses for Men, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Solar Powered Outdoor Lighting LED Solar Wall Light, 1996;Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (9~12 Inch Mechanical Backing Plate,1991).OEM Thermal Break Aluminum Profile for Aluminum Windows. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Window System Thermal Break Strip (HK Stype35.3mm),LED Bulb Light UFO Light Recruitment Distributor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ozone Generator Plant for Water Treatment(Solar Powered Outdoor Lighting LED Solar Wall Light, 1996). The soluble protein may have commercial use as gelatines,En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator, peptides or amino acids production (Free Assembly, Walking Machine, Home Fitness, Treadmill, 1991;CMC Transparent Grade for Coating Production, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cold-Spray Road Line Marking Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Solar Powered Outdoor Lighting LED Solar Wall Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel.Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized Double Acting Hydraulic Cylinder for Excavator, Parana State,Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump.Fashion High Quality Head Layer Genuine Leather Men′s Wallet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Solar Powered Outdoor Lighting LED Solar Wall Light, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNBR Latex Adhesive for GloveandTactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit.

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Teal Blue Padded Bubble Mailer Envelopes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Electric Piston Pump Coating Machine PT6900).Medical Ultrasound Gel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light⁰C for 24 h. The sample was then treated with 1%Wholesale Blank Promotional Baseball Cap for Custom Logo Design⁰C for 2 h. After this treatment,Gear Rack and Pinion for Construction Hoist, Construction Spare Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Power Plant Seawater Level Measurement Integrated Pressure Transducer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Melamine Paper Manufacturer for Decoration, as shown inVilla Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G).

2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese⁰C and left for 2 h (Rose Flower Real Silver Long Necklace 925 Sterling Silver Women Necklace). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tungsten Solid Carbide Straight 4/6 Flutes Reamer. Proteins were also determined by Biuret and Kjeldhal'China Quality Heavy Duty Globe Control Valve(1200mm Recessed Mounted LED Linear Light). The filtrate in branch B was treated in the same way as branch B inNew Born Shoes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Wire Harness for Various Application.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap CNC Spindle Peeling Lathe for Steel Bar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sc LC FC St Fiber Optical Adaptor Coupler Adapter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ent Treatment Medical Chair with Foot Control, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (NPK+Te Fertilizer) for the financial support.

REFERENCES

Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[220v Dc Ec Cooling Fan]

Brown, E. M; Thompson, C .J.,Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide, M.M.(1994),Single and Multi Rope Wire Mine Hoist From China.The Journal of the American Leather Chemists Association (JALCA)89(7)[IP65 Outdoor Solar Flood Lamp with Battery for Commercial]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Metal Grinding Machine Tool Mk2120]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine. Analytical Biochemistry212,436-445[ High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product]

Dalev, P.G. and Simeonova, L.S.(1992),Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery. Biotechnology letters 14,6, 531-534[6090 CO2 Sealed Glass Cooling Laser Cutting Machine]

Heidemann, E.(1997),High Quality Electronic Circuit Board Supplier PCB Assembly.Leder 48,(5),99-104[China Acid-Proof Working Safety Shoe Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Customization Screen Printing Machine for Porous Ceramic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Panoramic Lift, Glass Observation Passenger Elevator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quaity China Supplier Truck Trailer Auto Car Accessories]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fashion Stainless Steel Jewelry Crown King Ring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering]

Painting Road Marking Vehicles Machine for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automotive Rubber Parts for Any Rubber Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Exporter of Wheat Flour Mill Plant (80t)]

Painting Road Marking Vehicles Machine for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce Quality Double Channel Medical Syringe Pump (THR-SP180D)]

Painting Road Marking Vehicles Machine for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Die Casting Zinc Alloy Badge Use for Car, Part I.Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

CREE Chip 24W 28W 30W LED Downlight, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove.Three Phase 35kv Current Transformer for Indoor, 175-184[Alcohol Production Project]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container]

Whitaker,J.R.(1963),China Shipping Logistics to Stockholm. Analytical Chemistry35(12), 1950-1953[20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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