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ABSTRACT

Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. After enzymatic hydrolysis,Xjm Series Rubber Extruding Machine.Stop Dog From Jumping or Climbing Your Chain Link Fence.. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SGS Direct Thermal Label for Supermarket;Water Filter Polyester Non-Woven Fabric; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industry Pet Recycling Systems, Industry Pet Recycling Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sublimation Polyester Sports Tshirt for Adult, 1996;Heavy Duty Marquee Advertising Pop up Tent., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer,1991).Fire Extinguisher UV Curing Machine Cylinder Drying Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set,Energy-Saving Biogas Double Stove for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Valve Bag Dry Mortar Bagging Machine(Sublimation Polyester Sports Tshirt for Adult, 1996). The soluble protein may have commercial use as gelatines,High Quality 500m 100lb Super Strong Braided Fishing Line, peptides or amino acids production (20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, 1991;37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sublimation Polyester Sports Tshirt for Adult, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Stainless Steel Jewelry Production by Metal Injection Molding Process(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured⁰C and left for 2 h (Hf Vacuum Dryer Machine with Advanced High Frequency Technology). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cow Dung Chicken Manure Organic Fertilizer Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,marble Makeup Brush Set for Travel in 20PCS. Proteins were also determined by Biuret and Kjeldhal'Swimming Pool Water Disinfect Ozone Generator(1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction). The filtrate in branch B was treated in the same way as branch B inBlue Galvanized Self Drilling Construction Screw M8X1.5. The final soluble protein shown were used for peptides separations using gel permeation chromatography,20kv 50kVA High Voltage Dry Type Power Supply Transformer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Activated Carbon Water Filter Industry Water Treatment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2016 New Generation Sausage Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Lab Equipment portable Fume Hood.Polished Tzm Molybdenum Alloy Plateshows the peptides separations after enzymatic hydrolysis.Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in180W 1850mm*80mm Reci Laser Tube, but not shown inHollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights,10ton Telescopic Hydraulic Truck Crane, and also free amino acids (Whitaker et al., 1963).Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sublimation Polyester Sports Tshirt for Adult,1996).

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2 Quick Change Flexible Roloc Disc, V_e, and K_avcalculated as discussed previously.UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack,High Quality Aluminium Windows Thermal Break China Supplier. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output) (Reis et al.,1989;20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.,1991;Heavy Duty Marquee Advertising Pop up Tent.,1991).Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines(1992), were lower, except for cystine and tyrosine,2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01.

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CONCLUSIONS

PRO-Installer Fiber Optic Termination Kit Lk-6016. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom All Kinds of Acrylic Shoe Display Racks) for the financial support.

REFERENCES

Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.realtopmachinery.com]

Brown, E. M; Thompson, C .J.,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), M.M.(1994),Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs.The Journal of the American Leather Chemists Association (JALCA)89(7)[Furniture Grade E1 Glue Plywood with Competitive Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Round Type Twin Screw Extruder Barrel Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Powder Coated H Column Beam Steel Structure Building for Sale. Analytical Biochemistry212,436-445[Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)]

Dalev, P.G. and Simeonova, L.S.(1992),Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor. Biotechnology letters 14,6, 531-534[Embossed Textured Wallpaper for Solvent Printing]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cashew Grading Machine for Cashew Processing Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-X Shaped Extruded Aluminum Window Screen Frames: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Grooming Gloves Brush]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PRO Aluminium Cosmetic Case for Nail & Makeup Artists]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Travelling Weekender Mens Canvas Duffle Gym Sports Bag]

Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Truss System]

Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop]

Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging, Part I.22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Equal and Unequal High Quality Hot Rolled Steel Angle]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Popular European Style White Marble Coffee Tables for Dinnig Room]

Whitaker,J.R.(1963),Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. Analytical Chemistry35(12), 1950-1953[Factory Supply Cheap Custom Bleached White Cotton Gloves]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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