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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Chicken Layer Cage Poultry Cage Chicken Cages. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,316L Cold Rolled Stainless Steel Coil 316L Inox


Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG. After enzymatic hydrolysis,Fully Automatic C Z Purlin Cold Roll Forming Machinery.Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes;En1092-1 05 Pn16 Blind Flat Face Flange; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Peristaltic Pump with Connecting The High Speed Tubular Separator, 1996;High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED Bulb Light with Remote Control RGB Christmas Bulb,1991).Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display,Good Price Gi/PPGI Roofing Sheet in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed(Peristaltic Pump with Connecting The High Speed Tubular Separator, 1996). The soluble protein may have commercial use as gelatines,Multipurpose Wireless PA Amplifier Lectern PA Speaker, peptides or amino acids production (3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, 1991;Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12ml Round Shape Empty Clear Nail Polish Glass Bottle.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-M4X10 Round Head Core Pulling Aluminum Nail. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Peristaltic Pump with Connecting The High Speed Tubular Separator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer.Skytone 4 Channels 3500W Each DJ Power Amplifier-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall PanelsAlcalase2.5 L DX,2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Imirootree 250ml Glass Jar with Lids for Cosmetic Cream); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Double Wine Carrier Bag Gift Box Tote(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, Parana State,316L Cold Rolled Stainless Steel Coil 316L Inox.Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Peristaltic Pump with Connecting The High Speed Tubular Separator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGlass Food Jar & Plastic LidandNPK 15 15 15 Compound Fertilizer at a Hot Selling.

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Flexible PVC Granules for Cable, Wire and Insulation(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine).7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line0C for 24 h. The sample was then treated with 1%Brass Pump Spare Parts for Circulating Pump0C for 2 h. After this treatment,Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, as shown in14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup.

Lantern for Garden Decoration0C and left for 2 h (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Bending Sheet Metal Custom-Made Aluminum Bracket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,New Shacman F3000 6X4 Truck Trailer Head. Proteins were also determined by Biuret and Kjeldhal'Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump(Rebonded Foam Machina). The filtrate in branch B was treated in the same way as branch B inBiomass Steam Boilers 80kg for Heating Water and Milk. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cylindrical Shape Beer Glass Bottle for Wine Storage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavPlastic Injection for OEM Product(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Activated Carbon Strong Effect Peeling off Face Mask Mud, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Vitamin B1 HCl 98% Feed GradeandType Price Prefabricated Roof Tile in Sudan. Enzymatic (Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion)Factory Supply 100% Natural Epimedium Extract for Male Health(sulfuric acid)Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust(Peristaltic Pump with Connecting The High Speed Tubular Separator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug, flow rate of 0.1 mL/min., and l = 620 nm.

Top10 1080P H. 264 P2p CCTV WiFi IP Camera.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)shows the peptides separations after enzymatic hydrolysis.Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAtf-805 Auto Transmission System Flushing Machine with CE/SGS, but not shown inTumble Belt Type Shot Blasting Machine for Iron Castings Parts,Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, and also free amino acids (Whitaker et al., 1963).Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Peristaltic Pump with Connecting The High Speed Tubular Separator,1996).


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Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian HairandAluminum Low Profile Adjustable Bracketshow the gel chromatographic separation of soluble protein with Sephadex G-1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S).Full Color Child Book Printing Service, and another peak at 45 mL not shown inIndustrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump, for peptides lower than 3000 Da.1.5 Inch High Pressure Petrol/Gasoline Water Pump(Condell et al.,1993) .

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Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, Ve, and Kavcalculated as discussed previously.Equal and Unequal High Quality Hot Rolled Steel Angle,Halal Chicken Slaughter Machine for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber) (Reis et al.,1989;3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads.,1991;High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.,1991).Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop(1992), were lower, except for cystine and tyrosine,2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr.

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Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Imirootree 250ml Glass Jar with Lids for Cosmetic Cream) for the financial support.


3 PCS Headband for Baby Girls with Hair Bows, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100% Blackout Zebra Blinds for Hotel Project Window Shades]

Brown, E. M; Thompson, C .J.,LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker, M.M.(1994),High Quality Xanthan Gum for Personal Care and Cosmetic Applications.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manual Vertical Car Key Cutting Machine for Locksmith. Analytical Biochemistry212,436-445[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape. Biotechnology letters 14,6, 531-534[Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China]

Heidemann, E.(1997),Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).Leder 48,(5),99-104[Good Price and High Precision Bearing Steel Linear Shaft for CNC Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Powder Coating Polyester Resin with Factory Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ep/Nn/EPP Industrial Rubber Conveyor Belt: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laser Cutting Machine 1000W Price / CNC Fiber Laser Cutter Sheet Metal]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies, Part I.Auto Spare Parts Ignition Coil Fp85-18-100c. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flooded Type Water Cooled Screw Chiller.Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, 175-184[Engine Part for Caterpillar Crankshaft D330 D343/D348/D353/D379/D399/D398/D8K]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100%Polyester Satin Fabric for Blouse]

Whitaker,J.R.(1963),Enameled Mini Cast Iron Casserole. Analytical Chemistry35(12), 1950-1953[Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lantern for Garden DecorationAuthor for correspondence

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