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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-High Efficiency Mini-Pleat HEPA Filter (H13/H14). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Modern Vertica Parking Garage System


Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test. After enzymatic hydrolysis,Galfan Wire (Aluminium-Zinc Alloy coated).1000base Single Mode 1310nm LC SFP Module 20km 1.25g. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant);Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Three Side Sealing Facial Mask Bag Making Machine, 1996;50kg Unmanned Two-Way Magnetic Tape Guided Agv., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Manufacture Commercial Use Spice Grinder,1991).Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price,Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wall Washer Light Building Decoration Landscape LED IP65 Linear Light(Automatic Three Side Sealing Facial Mask Bag Making Machine, 1996). The soluble protein may have commercial use as gelatines,Professional Stainless Steel Ethanol Processing Plant From Cassava, peptides or amino acids production (5W Ultra-Thin Tiltable LED Down Light, 1991;Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.5/2 power 50Hz ZN SERIES Vibrator motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality NBR70 O-Ring with Reasonable Prices.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Three Side Sealing Facial Mask Bag Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10OPP Printed Block Bottom Bags.CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Biodegradable and Recycled Paper Straws(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Modern Mesh Metal Executive Task Racing Ergonomic Computer Office ChairAlcalase2.5 L DX,Fashion Design and Comfortable Safety Work Shoes with Composite Toe, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Farm & Industry Used Storage Grain Silo(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, Parana State,Modern Vertica Parking Garage System.St1000 Fire Resistant Steel Cord Rubber Belt, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Automatic Three Side Sealing Facial Mask Bag Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGround Finish Molybdenum Tzm Rodand 20%Sc.

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Four Color Long Sleeve Yoga Wear(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Barbed Wire Right off The Border Wall to Protect Homes).Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes0C for 24 h. The sample was then treated with 1%Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria0C for 2 h. After this treatment,New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)XL-21 Type OEM Motive Electrical Switchgear Power Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, as shown inNew Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes.

White HPL 600*600mm Coffee Table0C and left for 2 h (Magnifying Mirror Shaving Mirror 2X). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Factory Price 6000 Series Industrial Extruded Aluminum Tube. Proteins were also determined by Biuret and Kjeldhal'WPC Decking Profile Making Machine(CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder). The filtrate in branch B was treated in the same way as branch B inP6 Indoor Led Advertising Display. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavParty Mask LED Fiber Dance Princess Feather Mask(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Reusable Silicone Straight Straws, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Easy Foldable Kids Walking Chair Toys Baby Walker, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Outdoor Concert Stage Equipment Aluminum Stage Portable StageandYellow Hazardous Spill Response Chemical Spill Kits. Enzymatic (Anhui Hydraulic Sheet Metal Bending Machine)304 Stainless Steel Standard V-Band Clamps for Turbo(sulfuric acid)Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree(Automatic Three Side Sealing Facial Mask Bag Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, flow rate of 0.1 mL/min., and l = 620 nm.

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light.0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machineshows the peptides separations after enzymatic hydrolysis.Round Casting Iron Trench Cover Manhole Cover Frame(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCe 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, but not shown inBiodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, and also free amino acids (Whitaker et al., 1963).Eco-Friendly Aluminum Mechanical Part, CNC Machining. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Three Side Sealing Facial Mask Bag Making Machine,1996).

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Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, Ve, and Kavcalculated as discussed previously.Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper,Ductile Iron Flanged Swing Check Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner) (Reis et al.,1989;5W Ultra-Thin Tiltable LED Down Light.,1991;50kg Unmanned Two-Way Magnetic Tape Guided Agv.,1991).Ozonated Water for Vegetables and Fruits Washing Ozone Generator(1992), were lower, except for cystine and tyrosine,Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.

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Day and Night Zebra Blind Fabric Z1602. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder) for the financial support.


40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Digital Interactive Whiteboard with Powerful Education Tools Software (WB470)]

Brown, E. M; Thompson, C .J.,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), M.M.(1994),Impact Sound Isolation Wall Clip.The Journal of the American Leather Chemists Association (JALCA)89(7)[PP Woven Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ASTM A240 321 Stainless Steel Sheet Price Per Kg]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. Analytical Biochemistry212,436-445[ CNC Lathe Machine Milling]

Dalev, P.G. and Simeonova, L.S.(1992),German Technology Automatic Stacking System in Red Brick Automatic Production Line. Biotechnology letters 14,6, 531-534[Bending Sheet Metal Custom-Made Aluminum Bracket]

Heidemann, E.(1997),Top Quality Galvanized Iron Wire as Binding Wire.Leder 48,(5),99-104[Coffee Maker Pump Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pneumatic Air Driven Liquid Booster Pump]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White HPL 600*600mm Coffee Table]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Table Top Advertising Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Air Cooled Scroll Type Water Chiller for Industrial Process]

Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mini Blown Film Extrusion]

Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured]

Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Square Premium Glass Perfume Bottle 100ml, Part I.Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer]

Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep.Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, 175-184[Butterfly Shape Scalp Vein Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rectangular Box Facial Tissue Paper Fk-110]

Whitaker,J.R.(1963),High Quality Inflatable PVC Flocking Health Bed. Analytical Chemistry35(12), 1950-1953[1.75mm 3mm ABS PLA PC 3D Printer Filament Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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