ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Heat Treated Welding Protection Glassfiber Cloth Ht800. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring

ABSTRACT

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. After enzymatic hydrolysis,Heat Pump Non-Programmable HVAC Room Thermostat.Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable;Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Injector Pump Calibration Test Bench, 1996;ASME/ASTM Butt-Welding Stainless Steel Plate Flange., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank,1991).Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polyester / Polypropylene Woven Geotextile for Road Construction,Granite Stone Jaw Crusher of Mining Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor(Injector Pump Calibration Test Bench, 1996). The soluble protein may have commercial use as gelatines,Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, peptides or amino acids production (The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, 1991;Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale All Kinds Cheap Plush Animal Baby Slippers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Injector Pump Calibration Test Bench, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3 Layer Round Tea Tin with Air Tight Lid, Parana State,Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring.China PA System Power Amplifier with Enough Rated Power, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Injector Pump Calibration Test Bench, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePVC/PP/PE Twin Screw Plastic Granules Extruding Granulator MachineandFood Grade Stainless Steel Milk Dairy Equipment for Factory.

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Export Grade Citric Acid Monohydrate as Detergent(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chinese Jbcz Handy Operation Threading Machine and Coupler).Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Familu Use Mini Small Pig Feed Pellet Mill⁰C for 24 h. The sample was then treated with 1%3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light⁰C for 2 h. After this treatment,Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hairdressing Equipment Takara Belmont Barber Chair High Quality, as shown inFashion Design and Comfortable Safety Work Shoes with Composite Toe.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rectangular Box Facial Tissue Paper Fk-110. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.AAC Block Production Line / AAC Equipment Manufacturing/ Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Q32 Series Tumble Belt/Crawler Shot Blasting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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U. S. Type Drop Forged Wire Rope Clip, V_e, and K_avcalculated as discussed previously.Humizone Seaweed Customized Organic Liquid Fertilizer,Zipper Fabric Travel Passport Wallet Organiser. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sleeved Wire Harness for Industry Equipment) (Reis et al.,1989;The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine.,1991;ASME/ASTM Butt-Welding Stainless Steel Plate Flange.,1991).Hot Sale Instax Mini Camera Korea Style Photo Album(1992), were lower, except for cystine and tyrosine,Aluminum Low Profile Adjustable Bracket.

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CONCLUSIONS

Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar) for the financial support.

REFERENCES

High Quality Acrylic Beer Display Racks with LED, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Molybdenum Alloy Plate]

Brown, E. M; Thompson, C .J.,Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C), M.M.(1994),Baby Feeding Chair Plastic Chair for Feeding Hebei China.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle. Analytical Biochemistry212,436-445[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Injection Molding for Auto Parts. Biotechnology letters 14,6, 531-534[Modern American Style Beech Solid Wood Kitchen Cabinet]

Heidemann, E.(1997),Round Type Twin Screw Extruder Barrel Manufacturer.Leder 48,(5),99-104[Lab Homogenizing Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Water Treatment Anthracite, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ASA-UPVC Anti-Corrosive Composite Roof Tile.Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System. USP/SP[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sexy Mesh Transparent Female Running Tank Top with Hood Custom: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Frame Hydraulic Press Machine for Bending Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Type 0242 IP67 220V-415V 6h 3p E 3pin 32A Industrial Plug]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Familu Use Mini Small Pig Feed Pellet Mill]

Color Cardstock Color Paper Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cherry Wooden Grain PVC Film in Furniture]

Color Cardstock Color Paper Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Color Cardstock Color Paper Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Home and Office PVC Vinyl Flooring Conductive Plank Flooring, Part I.Precision Engineering Parts Bearing Support Unit for Ball Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug]

LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man.Silicone Cake Mold Sinle Cupcake DIY, 175-184[8wall Polycarbonate Sheet 35mm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers]

Whitaker,J.R.(1963),Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting. Analytical Chemistry35(12), 1950-1953[Air Vent Diffuser Adjustbable Air Well and Jet Diffuser]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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