ARTICLES

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ABSTRACT

Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing. After enzymatic hydrolysis,New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238.Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Auto Spare Parts Ignition Coil Fp85-18-100c;vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flooded Type Water Cooled Screw Chiller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, 1996;Enameled Mini Cast Iron Casserole., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Tomato Sauce Making Machine,1991).Vitamin B1 HCl 98% Feed Grade. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2018 TPR Clean Teeth Training Dog Pet Chew Toy,Good Quality Zinc Plated Slotted Hex Head Roofing Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine(Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, 1996). The soluble protein may have commercial use as gelatines,5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof, peptides or amino acids production (Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer, 1991;High Quality Orthopedic Fiberglass Splint, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Amazon Hot Selling Kids Playing Pop up Goal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Frame Hydraulic Press Machine for Bending Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3.5-7.0g Ozone Generator Purification ---Commercial. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Baby Feeding Chair Plastic Chair.Reflective Hand Hold Thermoplastic Road Marking-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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JIS S Type Cast Iron Water Filter Dn25(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Multi-Function Small Tree Digging Shovel/Tree Machine).Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factor Direct Adult Sex Toy Doll for Male Sex⁰C for 24 h. The sample was then treated with 1%C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L⁰C for 2 h. After this treatment,Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Worm Gear Fluorine Lined Butterfly Valve Flanged Type, as shown inCustom Stainless Steel Double Small Torsion Spring.

H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera⁰C and left for 2 h (1 Ton Chain Pulley Block with Geared Trolley). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Poultry House, Livestock, Poultry Farm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. Proteins were also determined by Biuret and Kjeldhal'Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2(High Quality Spooling Pet Polyester Mylar Film for Cable Material). The filtrate in branch B was treated in the same way as branch B inLed Slim Panel Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Black Slim PU Leather Passport Travel Wallet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.3KG Fire breaker for kitchen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Korean Integrated CTO Activated Carbon Filter for Water Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow.Chemical Product Sodium Gluconate Used for Concrete Admixtureshows the peptides separations after enzymatic hydrolysis.Wheel-Type-Carriage Precision Heavy Duty Linear Guide(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUs Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, but not shown inHigh Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit,Double-Offset Double Flanged Stainless Steel Butterfly Valve, and also free amino acids (Whitaker et al., 1963).Automotive Acrylic Wholesale Masking Tape Factory Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday,1996).

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CONCLUSIONS

12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine) for the financial support.

REFERENCES

Parts for Crane Outrigger Hydraulic Oil Cylinder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Design Outdoor Indoor Decoration Digital Graphic Water Curtain Fountain]

Brown, E. M; Thompson, C .J.,Boned Polyester Woven Faux Suede Sofa Fabric, M.M.(1994),Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making.The Journal of the American Leather Chemists Association (JALCA)89(7)[Transparent Color Stretch Wrap Film Manual Operate Wrap Film]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Motor Bearing Automobile Bearing Self-Aligning Ball Bearing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20 Bottles Electric Wine Display Cooler. Analytical Biochemistry212,436-445[Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag]

Dalev, P.G. and Simeonova, L.S.(1992),High-Efficient Htk Walnut Shell Filter for Water Purification. Biotechnology letters 14,6, 531-534[Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter]

Heidemann, E.(1997),Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576).Leder 48,(5),99-104[m.ok-agv.net]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale 30W LED Down Lights with Budget Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Trendy High Quality 925 Stering Silver Tennis Glod Charm]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10kv Outdoor Transformer Substation]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets]

FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kwikstage Scaffolding System-Diagonal Brace-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine]

FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter]

FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:360 Rotating Automatic Touchless Car Wash Equipment with Drying System, Part I.48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless]

Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump.Sell Rubber / EVA / PE/PVC Foam Board, 175-184[Color Changing Sticker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automotive Acrylic Wholesale Masking Tape Factory Price]

Whitaker,J.R.(1963),Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. Analytical Chemistry35(12), 1950-1953[Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Trailer RV Vision SecurityAuthor for correspondence