ARTICLES

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ABSTRACT

Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens. After enzymatic hydrolysis,Custom Design Plush Panda Animal Slippers Comfort Foam Support by.Best 6-Axis Electric Screwdriver Machine Robot. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts;Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FTTH Embedded Type Sc Fiber Optic Fast Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multi-Function Small Tree Digging Shovel/Tree Machine, 1996;Automatic L Bar Sealer Shrink Tunnel Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Automatic Carbonated Water Filling Full Line,1991).2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Superior Watertightness JS Polymer Cement Waterproof Coating for Basement,PS 200 Upper Discharge Centrifuge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew(Multi-Function Small Tree Digging Shovel/Tree Machine, 1996). The soluble protein may have commercial use as gelatines,Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, peptides or amino acids production (Electrosurgical Generator High Frequency Electricity Kinfe, 1991;Viton O Ring Kit with Excellent with Oil Resistance, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;One Step HBV 5 in 1 Rapid Test., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Summer Fresh Landscaping Turf Lawn. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multi-Function Small Tree Digging Shovel/Tree Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pipe Sheet Laser Cutter with Tube Cutting.Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill, Parana State,19mic Black Cast PE Stretch Film for Pallet Wrapping.Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Multi-Function Small Tree Digging Shovel/Tree Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module ScreenandMedium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector.

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Baking Soda 99.8% Min Sodium Bicarbonate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Felt Belt for Textile Compactor).BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine⁰C for 24 h. The sample was then treated with 1%6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower⁰C for 2 h. After this treatment,Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10g Non-Woven Fabric Silica Gel Desiccant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Soosan Excavator Hydraulic Hammer for Construction Demolition, as shown in100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve.

Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover⁰C and left for 2 h (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile. Proteins were also determined by Biuret and Kjeldhal'Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950(Wall Washer Light Building Decoration Landscape LED IP65 Linear Light). The filtrate in branch B was treated in the same way as branch B inDiving Helmet Brass. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Cacao Bean Grinder Cocoa Butter Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Concrete Aluminum Formwork System Aluminium Formwork⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

marble Makeup Brush Set for Travel in 20PCSandHigh Quality Spooling Pet Polyester Mylar Film for Cable Material. Enzymatic (Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming)Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir(sulfuric acid)2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo(Multi-Function Small Tree Digging Shovel/Tree Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant, flow rate of 0.1 mL/min., and l = 620 nm.

150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels.Round Casting Iron Trench Cover Manhole Cover Frameshows the peptides separations after enzymatic hydrolysis.Best Design and Nice Price All in One Solar Street Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared iniPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, but not shown inIndependent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board,Customized Large Ss Stamping/ Bending Sheet Metal Parts, and also free amino acids (Whitaker et al., 1963).High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multi-Function Small Tree Digging Shovel/Tree Machine,1996).

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CONCLUSIONS

Polished Tzm Molybdenum Alloy Plate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toast Stamp Loaf Bread Tin Pan (ZMA)) for the financial support.

REFERENCES

Iron Welded Gabion Basket Retaining Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Organic Food Grade Yeast Beta Glucan Powder]

Brown, E. M; Thompson, C .J.,Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, M.M.(1994),Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835. Analytical Biochemistry212,436-445[Type Price Prefabricated Roof Tile in Sudan]

Dalev, P.G. and Simeonova, L.S.(1992),FTTX Solution Provider Fiber Optical 12cores MPO Cassette. Biotechnology letters 14,6, 531-534[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Heidemann, E.(1997),Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer.Leder 48,(5),99-104[Professional Manufacturer HDPE Labyrinth Drip Belt Extruder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Explosion Proof Fog Cannon for Coal Plant, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Height Adjustable Cane.Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board. USP/SP[High Quality Aluminium Windows Thermal Break China Supplier]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Large Batches Equipoise Light Yellow Liquids: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Cap Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122]

Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Custom Football Net Soccer Goal Net for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow]

Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making]

Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO, Part I.Load 1600kg Small Machine Room Bed Elevator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DIN Standard Gratings/Gavlanized Steel Grating]

Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service.New Invention 2017 Male & Female Stainless Steel Quick Coupling, 175-184[Fiber Optic Transceiver]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin]

Whitaker,J.R.(1963),Driving System for Hybrid Stepper Motor Dm860A. Analytical Chemistry35(12), 1950-1953[En1092-1 05 Pn16 Blind Flat Face Flange]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

10g Food/Pharmaceutical Desiccant Silica Gel for Moisture AbsorbingAuthor for correspondence