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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. After enzymatic hydrolysis,Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool.Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Personalized Coffee Mug Cup with Custom Logo, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, 1996;10 Pieces Customized Professional Makeup Brush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone,1991).Electrophoresis Assembly Infilltration Tank for Effluent Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,700mm RP HP UHP High Quality Graphite Electrode,Prefab Building Insulated Interior Concrete Partition Walls,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disk Filter System with Automatic Backwash Valves(100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, 1996). The soluble protein may have commercial use as gelatines,Eco-Friendly Recycling PS Foam Container Production Line, peptides or amino acids production (Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, 1991;Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jacquard Football Fans Acrylic Sports Warmer Scarf.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Small Blinking Battery Operated Luminary Plastic LED Tea Lights.Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading).Blasting Cleaning Shot Peeling Machine Tumble Belt Type(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine0C for 24 h. The sample was then treated with 1%Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap0C for 2 h. After this treatment,Chemical Plant Transfer Industrial Water Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Insecticidal Lamp with Solar Energy Frequent Vibration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286, as shown inClay Tiles Making Machine with Latest Technology.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavGood Quality PVC Plastic Ceiling for Wall Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CE Approved Manual Heat Press Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820), pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mango Drying Machine/Solar Fruit Drying Machine) for the financial support.

REFERENCES

2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cut out Iron Stamped Antique Silver Plated 3D Logo Medal]

Brown, E. M; Thompson, C .J.,Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine, M.M.(1994),Oak Laminate Grey Floating Cork Vinyl Plank Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Manufacturer Customized Microwave Ring Ferrite Magnet. Analytical Biochemistry212,436-445[Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody]

Dalev, P.G. and Simeonova, L.S.(1992),Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish. Biotechnology letters 14,6, 531-534[Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)]

Heidemann, E.(1997),Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody.Leder 48,(5),99-104[Hairline Stainless Steel Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Car Seat Belt Buckle Mold Maker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3 Colors Tactical Shooting Hunting Camouflage Clothes Suit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Tracked Transport Loader Garden Mini Crawler Loader Agricultural Transport Loader]

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DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry, Part I.Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Invention 2017 Male & Female Stainless Steel Quick Coupling]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention]

Whitaker,J.R.(1963),Adjustable Aluminum Solar Mounting System. Analytical Chemistry35(12), 1950-1953[8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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