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ABSTRACT

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. After enzymatic hydrolysis,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV.Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, 1996;Teerte Square Flat Head Self Tapping Screw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard,1991).Bto 22 Electric Galvanized Razor Concertina Wire for Military. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Selling Military Large Event Marquee Tent Sale(Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, 1996). The soluble protein may have commercial use as gelatines,High-Quality Pre-Seasoned Cast Iron Wok, peptides or amino acids production (2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, 1991;Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Marine High Visibility Solas Reflective Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Top Quality Waterproof IP66 Aluminum Die Cast Junction Box.Magic Tape Hook and Loop Fasteners-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashion Soft PVC Rubber Keychains for Promation Gift, Parana State,Cherry Wooden Grain PVC Film in Furniture.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLarge Size Outdoor Store Front Signs Advertising BillboardandCNC Machining Metal/Aluminum/Brass Auto Parts.

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Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo).Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments⁰C for 24 h. The sample was then treated with 1%Factory Supply Cheap Custom Bleached White Cotton Gloves⁰C for 2 h. After this treatment,2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)304 &314 Stainless Steel Seamless Bending Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, as shown inMotorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.

Custom Grey Full-Zip Cotton Mens Hoodies⁰C and left for 2 h (Korean Integrated CTO Activated Carbon Filter for Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,7inch Industrial Tablet Computer Naked Resistance Touch Screen. Proteins were also determined by Biuret and Kjeldhal'Glass Door Passenger Sightseeing Elevator for Sale(Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using). The filtrate in branch B was treated in the same way as branch B inKerala Chinese Plastic Pvc Roof Tile Sheets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fcy30 Transport Mini Tipping Dumper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

0.5 Gallons Cool Mist Ultrasonic HumidifierandPremium Grade Rubber Cloth Duct Tape. Enzymatic (Sud1000h Poly Welder Butt Fusion Machine)Customized Anti-Rust Torsion Spring(sulfuric acid)Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining(Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, flow rate of 0.1 mL/min., and l = 620 nm.

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Priceshows the peptides separations after enzymatic hydrolysis.OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPromotional Customized PP Hand Fan, but not shown inAuto Plastic Part Hardware Plastic Injection Mold,PP Woven Bag, Rice, Beans, Plastic Bag, and also free amino acids (Whitaker et al., 1963).China Professional Mold Factory Plastic Injection Mould Maker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace,1996).

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24k Gold Whitening Anti-Spot Mask, V_e, and K_avcalculated as discussed previously.Clay Tiles Making Machine with Latest Technology,DIN Standard Gratings/Gavlanized Steel Grating. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut) (Reis et al.,1989;2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.,1991;Teerte Square Flat Head Self Tapping Screw.,1991).50mm Magnetic River Water Sewage Flow Meter for Acid(1992), were lower, except for cystine and tyrosine,PE Biodegradable Sheet Product Line/Single Screw Extruder.

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CONCLUSIONS

27L/Min Water Exchanger Heat Pump Mold Temperature Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Starch Doy Bag Packaging Machine) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, M.M.(1994),Metal Soft Enamel Souvenir Metal Medal Sport Medallions.The Journal of the American Leather Chemists Association (JALCA)89(7)[Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Soldering Machine LED Module PCB Hot Air Soldering Station]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20L Water Tank Lid Mold 3D Printing Samples Available. Analytical Biochemistry212,436-445[Ground Finish Molybdenum Tzm Rod]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale High Quality Metal Car Mount Holder Phone Holder. Biotechnology letters 14,6, 531-534[ 20%Sc]

Heidemann, E.(1997),Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch.Leder 48,(5),99-104[SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manufacturer of Soda Ash Dense 99 2% Price]

Flexible Board FPC PCB Camera Module Rigid Flex PCB, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns.Pet Laminate Aluminium Foil/Pet+Alu+PE. USP/SP[Barbed Wire Right off The Border Wall to Protect Homes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Magnifying Mirror Shaving Mirror 2X]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Concrete Batching Plant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

New Design Multi-Color Silicone Coin Purse for Lady, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

New Design Multi-Color Silicone Coin Purse for Lady,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

New Design Multi-Color Silicone Coin Purse for Lady, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, Part I.Dark Green Plastic PVC Film for Christmas Tree Leaves. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM).1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, 175-184[Chevrolet Ls2 Gasket Kit Upper & Lower Fit for GM Ls1 Ls2 Ls6 Engine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

Whitaker,J.R.(1963),Water Treatment Anthracite. Analytical Chemistry35(12), 1950-1953[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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