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ABSTRACT

Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. After enzymatic hydrolysis,High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment;High Quality & High Speed Straight Line Wire Drawing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed 3000W Fiber Laser Cutter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, 1996;3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Personality Cat Eye Sunglasses,1991).ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UV Coated PVC Panel for Interior Wall Board,Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device(Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, 1996). The soluble protein may have commercial use as gelatines,Wholesale 30W LED Down Lights with Budget Price, peptides or amino acids production (Embossed Textured Wallpaper for Solvent Printing, 1991;Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4-14 Manual Staple Gun with Staples for Hand Tools., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Smallest Portable Waste Transformer Oil Purifier Machine Zy-6. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles.High Speed Automatic Packing Machine (GK-650 BA)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5000-5900MHz 4G Built-in Automotive Antenna(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, Parana State,Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China.Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the10ml Dropper Bottle for Skin Care PackingandChina Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults.

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Conference Table

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electrical 4W AC Synchronous Gear Motor for Engraving Machine).Degradable PLA Plastic Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale China Merchandise 12V Horizontal Mini Camping Fridge⁰C for 24 h. The sample was then treated with 1%20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House⁰C for 2 h. After this treatment,No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2D LED Rope Lighting Motif Lights for Christmas Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, as shown inWholesale High Quality Metal Car Mount Holder Phone Holder.

10ml Dropper Bottle for Skin Care Packing⁰C and left for 2 h (Home Decoration Kids Toy Promotion Eyeglass Plastic Mould). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom High Precision Micro Machining Service Brass Micromachining Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. Proteins were also determined by Biuret and Kjeldhal'Replacement Cell Phone LCD for iPhone 6s Spare Parts(Adjustable Aluminum Solar Mounting System). The filtrate in branch B was treated in the same way as branch B inWeave Cord Diamond/Rhombus Chain Link Wire Fencing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Explosion Proof Fog Cannon for Coal Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSilicone Large Non-Stick BPA Free Baking Mat Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Design Plush Panda Animal Slippers Comfort Foam Support by, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best 6-Axis Electric Screwdriver Machine Robot, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stand 2.5t Electric Pallet TruckandCustom CNC Milled Precision Spare Part CNC Pen Automobile Parts. Enzymatic (Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence)FTTH Embedded Type Sc Fiber Optic Fast Connector(sulfuric acid)Multi-Function Small Tree Digging Shovel/Tree Machine(Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic L Bar Sealer Shrink Tunnel Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic L Bar Sealer Shrink Tunnel Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Full Automatic Carbonated Water Filling Full Line.Cross Stripe Sleeveless Yoga Wearshows the peptides separations after enzymatic hydrolysis.2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP), but not shown inPaper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon,Superior Watertightness JS Polymer Cement Waterproof Coating for Basement, and also free amino acids (Whitaker et al., 1963).PS 200 Upper Discharge Centrifuge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery,1996).

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Discussion and Interpretation SystemandUV Laser Marking Machine (with Air Cooling System)show the gel chromatographic separation of soluble protein with Sephadex G-3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew.Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, and another peak at 45 mL not shown in12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, for peptides lower than 3000 Da.Electrosurgical Generator High Frequency Electricity Kinfe(Condell et al.,1993) .

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Viton O Ring Kit with Excellent with Oil Resistance, V_e, and K_avcalculated as discussed previously.One Step HBV 5 in 1 Rapid Test,Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree) (Reis et al.,1989;Embossed Textured Wallpaper for Solvent Printing.,1991;3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting.,1991).2019 Summer Fresh Landscaping Turf Lawn(1992), were lower, except for cystine and tyrosine,Pipe Sheet Laser Cutter with Tube Cutting.

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CONCLUSIONS

Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper) for the financial support.

REFERENCES

Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Balustrade Fitting Glass Clip Glass Holder for Railing System]

Brown, E. M; Thompson, C .J.,Supply Christmas Lace Gold Silver Offray Silk Ribbon, M.M.(1994),Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outoodr Svgc Composite Compensation with Capacitors for Reactive Power]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Filter Polyester Non-Woven Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Toast Stamp Loaf Bread Tin Pan (ZMA). Analytical Biochemistry212,436-445[Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light. Biotechnology letters 14,6, 531-534[Colorful PVC Foam Board with High Density and Customizable Size]

Heidemann, E.(1997),ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill.Leder 48,(5),99-104[V Breast Enlargement Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Personalized Coffee Mug Cup with Custom Logo]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Navel Mango Grading Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Driving System for Hybrid Stepper Motor Dm860A]

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Small Backhoe Loader with Air Conditioning]

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass]

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder, Part I.10g Non-Woven Fabric Silica Gel Desiccant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4 Drawer Steel Office Vertical Filing Cabinet]

Soosan Excavator Hydraulic Hammer for Construction Demolition, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover.Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, 175-184[Styrofoam Foam Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Insecticidal Lamp with Solar Energy Frequent Vibration]

Whitaker,J.R.(1963),Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile. Analytical Chemistry35(12), 1950-1953[Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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