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ABSTRACT

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. After enzymatic hydrolysis,Galvanized Square Wire Mesh, Metal Window Screen.Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Children Bear English Story Book Printing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flexible Board FPC PCB Camera Module Rigid Flex PCB, 1996;High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cherry Wooden Grain PVC Film in Furniture,1991).Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV,Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump(Flexible Board FPC PCB Camera Module Rigid Flex PCB, 1996). The soluble protein may have commercial use as gelatines,Linen Quiltcover Set with Quilting, peptides or amino acids production (2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, 1991;Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Teerte Square Flat Head Self Tapping Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bto 22 Electric Galvanized Razor Concertina Wire for Military. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flexible Board FPC PCB Camera Module Rigid Flex PCB, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Military Large Event Marquee Tent Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Top Quality Waterproof IP66 Aluminum Die Cast Junction Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (River Water Cleaning Sludge Sediment Machine Lamella Clarifier).Magic Tape Hook and Loop Fasteners(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool⁰C for 24 h. The sample was then treated with 1%Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)⁰C for 2 h. After this treatment,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Starch Doy Bag Packaging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), as shown inFrame Type Rubber Hydraulic Press Machine.

Fashion Soft PVC Rubber Keychains for Promation Gift⁰C and left for 2 h (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. Proteins were also determined by Biuret and Kjeldhal'Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes(Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor). The filtrate in branch B was treated in the same way as branch B inCalendar Manufacturer Cheap Price Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supply Cheap Custom Bleached White Cotton Gloves. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Grey Full-Zip Cotton Mens Hoodies, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase.OEM Gray Cast Iron Sand Casting Products with Universal Partsshows the peptides separations after enzymatic hydrolysis.Fcy30 Transport Mini Tipping Dumper(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, but not shown inNew Design Multi-Color Silicone Coin Purse for Lady,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, and also free amino acids (Whitaker et al., 1963).Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Flexible Board FPC PCB Camera Module Rigid Flex PCB,1996).

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Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, V_e, and K_avcalculated as discussed previously.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Slotter Sheet Metal V Grooving Machine From Professional Manufacturer) (Reis et al.,1989;2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life.,1991;High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing.,1991).OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic(1992), were lower, except for cystine and tyrosine,PP Woven Bag, Rice, Beans, Plastic Bag.

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CONCLUSIONS

China Professional Mold Factory Plastic Injection Mould Maker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine) for the financial support.

REFERENCES

Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Bags Packing Machine for Medical Cotton Roll Between 20 Grams to 1000 Grams. Medical Lap Making Machines Absorbent Cotton Carding Machine for Batt]

Brown, E. M; Thompson, C .J.,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, M.M.(1994),Industrial Carbon Steel Wire Hose Hydraulic Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-24k Gold Whitening Anti-Spot Mask. Analytical Biochemistry212,436-445[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

Dalev, P.G. and Simeonova, L.S.(1992),Clay Tiles Making Machine with Latest Technology. Biotechnology letters 14,6, 531-534[5000-5900MHz 4G Built-in Automotive Antenna]

Heidemann, E.(1997),DIN Standard Gratings/Gavlanized Steel Grating.Leder 48,(5),99-104[Engineered Ceramic Products for Chemical Industry]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

Chenille Microfiber Mop Head, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),50mm Magnetic River Water Sewage Flow Meter for Acid.PE Biodegradable Sheet Product Line/Single Screw Extruder. USP/SP[Vertical Type Automatic Box Erector Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Corrugated Steel Sheet Corrugated Steel Roofing Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-27L/Min Water Exchanger Heat Pump Mold Temperature Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Caulk Strip Trim Tape (38mm)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coral Towels]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress]

Felt Belt for Textile Compactor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile]

Felt Belt for Textile Compactor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heavy Duty Marquee Advertising Pop up Tent]

Felt Belt for Textile Compactor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Soft Enamel Souvenir Metal Medal Sport Medallions, Part I.20L Water Tank Lid Mold 3D Printing Samples Available. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5]

Wholesale High Quality Metal Car Mount Holder Phone Holder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch.High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, 175-184[Garden Hose Water Brass Quick Connect Fitting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Energy-Saving Biogas Double Stove for Sale]

Whitaker,J.R.(1963),Pet Laminate Aluminium Foil/Pet+Alu+PE. Analytical Chemistry35(12), 1950-1953[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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