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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Bio Media. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine

ABSTRACT

Platform Trolley with 150kg Capacity, 4 Castor Wheel. After enzymatic hydrolysis,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine.LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Alumina Ball with High Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Textile Fabric for Outdoor Sports Waterproof Backpack, 1996;White 4*8FT 18mm PVC Foam Board for Cabinets Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating,1991).Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Powerful Anti-Stress, Strong Flexibility Spoke Wire,Modern American Style Beech Solid Wood Kitchen Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hero Brand Polypropylene Bags Machine(PVC Textile Fabric for Outdoor Sports Waterproof Backpack, 1996). The soluble protein may have commercial use as gelatines,CNC Machining Metal/Aluminum/Brass Auto Parts, peptides or amino acids production (Surprise! Range Hood Free! ! ! Juice Cart, 1991;Chicken Layer Cage Poultry Cage Chicken Cages, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;316L Cold Rolled Stainless Steel Coil 316L Inox., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fully Automatic C Z Purlin Cold Roll Forming Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Textile Fabric for Outdoor Sports Waterproof Backpack, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:En1092-1 05 Pn16 Blind Flat Face Flange(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Good Price Gi/PPGI Roofing Sheet in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole).2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Multipurpose Wireless PA Amplifier Lectern PA Speaker⁰C for 24 h. The sample was then treated with 1%3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads⁰C for 2 h. After this treatment,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12ml Round Shape Empty Clear Nail Polish Glass Bottle, as shown inAluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W.

M4X10 Round Head Core Pulling Aluminum Nail⁰C and left for 2 h (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Skytone 4 Channels 3500W Each DJ Power Amplifier. Proteins were also determined by Biuret and Kjeldhal'Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price( Highly Stable Performance Disposable Blood Lancet with Cheap Price). The filtrate in branch B was treated in the same way as branch B inLab High Speed Dissolver Mixer (KFS-400W). The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avImirootree 250ml Glass Jar with Lids for Cosmetic Cream(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flexible PVC Granules for Cable, Wire and Insulation, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray BottlesandFlooded Type Water Cooled Screw Chillershow the gel chromatographic separation of soluble protein with Sephadex G-Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, and another peak at 45 mL not shown inHot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape, for peptides lower than 3000 Da.Cylindrical Shape Beer Glass Bottle for Wine Storage(Condell et al.,1993) .

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CONCLUSIONS

Type Price Prefabricated Roof Tile in Sudan. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding) for the financial support.

REFERENCES

Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Acne Removal LED PDT Korea Beauty Equipment LED Face Mask]

Brown, E. M; Thompson, C .J.,Factory Supply 100% Natural Epimedium Extract for Male Health, M.M.(1994),Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Design and Nice Price All in One Solar Street Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug. Analytical Biochemistry212,436-445[Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics]

Dalev, P.G. and Simeonova, L.S.(1992),Top10 1080P H. 264 P2p CCTV WiFi IP Camera. Biotechnology letters 14,6, 531-534[OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner]

Heidemann, E.(1997),Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture.Leder 48,(5),99-104[Sterilant Gas Mixtures 10% Eo and 90% CO2 From Dsw]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Aluminum Sheet Press Brake]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Step Motor for Intelligent Sanitary/3D Printer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Pure Teflon Rod with Best Chemical Corrosion Resistance (HY-P330R)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Swimming Pool Water Disinfect Ozone Generator]

Full Color Child Book Printing Service, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.5 Inch High Pressure Petrol/Gasoline Water Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing]

Full Color Child Book Printing Service,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input]

Full Color Child Book Printing Service, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, Part I.Equal and Unequal High Quality Hot Rolled Steel Angle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glass Door Passenger Sightseeing Elevator for Sale]

Whitaker,J.R.(1963),Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8. Analytical Chemistry35(12), 1950-1953[Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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