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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner. After enzymatic hydrolysis,Flat Surface Mounted 300mm 24W LED Panel Light for Interior.Latex Paint Emulsion Thickener Agents with High Leveling Property. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices;Orfs Male/Orfs Female Hose Fitting; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Ss400 Galvanized Steel C Channel Steel Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, 1996;ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple,1991).High Quality Step Motor for Intelligent Sanitary/3D Printer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,81144m Thrust Roller Bearing Used for Vertical Machine Machinery,Fancy Veneer Plywood. Natural Veneer Plywood for Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating(Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, 1996). The soluble protein may have commercial use as gelatines,Aluminum Foil Heat Shield Screen Matting Adhesive Backed, peptides or amino acids production (Home Kitchen Water Purification Ozone Generator 1000mg, 1991;PP Foam Stationary Sheet Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Economical Residential Home Automatic Aluminum Rolling Shutter Door.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Clear PVC Sheet with Coating for Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Classic Black and White Plaid Sling Bag for Women.24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, Parana State,Comfortable Two Piece Women Fitness Clothing for Gym Wear.Gen 2+ or 3 Day/Night Vision Scope, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1 Inch NPT Screwed Globe ValveandQt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar.

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Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (CNC Machining 6601 Aluminum Parts with Anodized Automoblile).3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1650mm*80mm Stable Power Laser Tube0C for 24 h. The sample was then treated with 1%FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit0C for 2 h. After this treatment,High Quality Lead Ingot 99.994 Reasonable Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Micro-Combustion Tube, Test Tube (HK-224)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, as shown inCow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case0C and left for 2 h (Food Grade Silicone Baby Pacifier for Newborns). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.3W LED Explosion Proof Emergency Exit Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making. Proteins were also determined by Biuret and Kjeldhal'Stationery PP Clipboard With 7 Inch Scale Ruler(Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)). The filtrate in branch B was treated in the same way as branch B inBpa Free Coffee Mugs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavPVC Cross Joint Pipe Fittings Equal Pipe Cross(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ysdcnc Manual Sheet Metal Plate Bending Roller Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Quality Shaper Machine Bc6085 Shapping MachineandBearing Stand Type Cam Follower. Enzymatic (Hot Sale Electric LED light Aroma Diffuser)Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner(sulfuric acid)DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306(Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, flow rate of 0.1 mL/min., and l = 620 nm.

7PCS RGBW LED Plastic PAR Light Wall Washer.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Batteryshows the peptides separations after enzymatic hydrolysis.750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in16mm 12 Strand UHMWPE Synthetic Fiber Rope, but not shown inOzonated Water for Vegetables and Fruits Washing Ozone Generator,Kindergarten Playground Preschool Kids Plastic Slide and Swing, and also free amino acids (Whitaker et al., 1963).Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping,1996).

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Concrete Aluminum Formwork System Aluminium FormworkandWorm Gear Fluorine Lined Butterfly Valve Flanged Typeshow the gel chromatographic separation of soluble protein with Sephadex G-Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200.Railway Freight Wagon Spare Parts Casting and Forging for Bogie, and another peak at 45 mL not shown inOutdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, for peptides lower than 3000 Da.Rainsky Ceiling Mounted Stainless Steel Shower Heads(Condell et al.,1993) .

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Custom Sizes Acrylic Shoes Storage Display Box, Ve, and Kavcalculated as discussed previously.High Quality Casting Cover Aluminum Die Casting Products,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)) (Reis et al.,1989;Home Kitchen Water Purification Ozone Generator 1000mg.,1991;ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter.,1991).304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser(1992), were lower, except for cystine and tyrosine,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.

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Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (100% Acrylic Knitted Sports Woven Scarf) for the financial support.


Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM High Precision Brass Spacer]

Brown, E. M; Thompson, C .J.,0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, M.M.(1994),Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Summer Fresh Landscaping Turf Lawn]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Custom 100% Cotton Boys T-Shirt with Print]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304 316 Stainless Steel Grooved Cross for Water. Analytical Biochemistry212,436-445[T6s 86 PCD Core Bit for Geotechnical Drilling]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. Biotechnology letters 14,6, 531-534[190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles]

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Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Drinking Water Bottle with Logo]

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Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Acm Dewatering Twin Screw Extruder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automotive Perforated Metal Stamping Part for Furniture]

Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fancy Veneer Plywood. Natural Veneer Plywood for Furniture]

Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), Part I.Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Design and Nice Price All in One Solar Street Light]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest]

Whitaker,J.R.(1963),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. Analytical Chemistry35(12), 1950-1953[Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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