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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster. After enzymatic hydrolysis,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122.Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing;UCP Color Army Camouflage Uniform Army Military Uniform Suit; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.0.5 Gallons Cool Mist Ultrasonic Humidifier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, 1996;Sanjing PVC Lining Layflat Fire Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar,1991).China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic CNC Glass Cutting Machine Glass Cutting Table,Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken(Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, 1996). The soluble protein may have commercial use as gelatines,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, peptides or amino acids production (Pet PP Rope and Broom Wire Drawing Machine, 1991;14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PTFE Seat Wafer Butterfly Valve Pn16., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Plain Makeup Bag Canvas Cosmetic BagAlcalase2.5 L DX,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, Parana State,Portable Electric Concrete Vibrator with 18000 High Rotation Speed.Factory Wholesale Qualited Polyester Padding Men Jacket, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np IntermediateandWomen Summer Swimsuit Chest Zip Bathing Suit.

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5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065).Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration0C for 24 h. The sample was then treated with 1%Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition0C for 2 h. After this treatment,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)River Water Cleaning Sludge Sediment Machine Lamella Clarifier's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polyester Coated Stainless Steel Ladder Type Metal Ties, as shown inCnm Single Strand Anchor Coupler.

2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device0C and left for 2 h (Fashion Design Underwear for Men Cotton Mens Boxer Briefs). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Copper Laminated Steel Strip T2/C11000. Proteins were also determined by Biuret and Kjeldhal'Halal Empty Gelatin Capsule for Medicine(Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel). The filtrate in branch B was treated in the same way as branch B inCustom Processing Aluminium Hardware Metal Machinery CNC Milling Turning Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gym Fitness Exercise Sporting Body Building Bicep Press. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavChinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


IEC 62196-2 Type 2 EV Charging 32A Male PlugandPolyester Dust Sport Gym Fabric Shoes Drawstring Bag. Enzymatic (Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet)1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price(sulfuric acid)Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine(Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, flow rate of 0.1 mL/min., and l = 620 nm.

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.10mm Red Green Yellow Warning China Supplier Signal Lampshows the peptides separations after enzymatic hydrolysis.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDurable Steel Retainer Plastic Road Safety Trench Cover, but not shown inAC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m,Plastic Food Box Container Covers by Injection Mould Mold Tool, and also free amino acids (Whitaker et al., 1963).304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates,1996).

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CE Approved Manual Heat Press MachineandCe RoHS FCC Shop Advertising Neon Illuminated LED Open Signshow the gel chromatographic separation of soluble protein with Sephadex G-Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans.Passenger Home Sightseeing Elevator Good Sales with Professional Technology, and another peak at 45 mL not shown inDIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water, for peptides lower than 3000 Da.Vacuum Stainless Steel Casting Products(Condell et al.,1993) .

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Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, Ve, and Kavcalculated as discussed previously.1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer,I-Tech 12000HD 2u Space Digital Professional Power Amplifier. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2016 New Generation Sausage Making Machine) (Reis et al.,1989;Pet PP Rope and Broom Wire Drawing Machine.,1991;Sanjing PVC Lining Layflat Fire Hose.,1991).Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer(1992), were lower, except for cystine and tyrosine,High Quality Silicone Reusable Food Storage Bag China Manufacturer.

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Polyurethane Gasket Foam Seal Dispensing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose) for the financial support.


Imee Print Customized Printing Agenda Printing Nb21, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manual6 Cylinders Injector Tester and Cleaner]

Brown, E. M; Thompson, C .J.,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, M.M.(1994),High Performance Multifunctional Crawler Mini Skid Steer Loader.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Wine Bottle Opener K020]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Slim PU Leather Passport Travel Wallet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. Analytical Biochemistry212,436-445[Replacement Cell Phone LCD for iPhone 6s Spare Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Chunke Portable UV Water Sterilizer for Water Purifier 150t. Biotechnology letters 14,6, 531-534[2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed]

Heidemann, E.(1997),Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress.Leder 48,(5),99-104[Yf-Sxbsjwkdt Double Arm Surgical Pendant(Go up Professional Manufacturer ICU Used Medical Column Pendant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory]

Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain.Xjm Series Rubber Extruding Machine. USP/SP[Diameter 2.68m Paragraphed Autoclave for AAC Bricks]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stop Dog From Jumping or Climbing Your Chain Link Fence.: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ferrite Core for Power Transformer (Ee16e)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heat-Proof Fire Resistant Silicone Coated Fiberglass Hydraulic Hose Guard]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PS 200 Upper Discharge Centrifuge]

SGS Direct Thermal Label for Supermarket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Filter Polyester Non-Woven Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture]

SGS Direct Thermal Label for Supermarket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets]

SGS Direct Thermal Label for Supermarket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industry Pet Recycling Systems, Industry Pet Recycling Line, Part I.Sublimation Polyester Sports Tshirt for Adult. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair]

Heavy Duty Marquee Advertising Pop up Tent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer.Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 175-184[China Stainless Steel 304 Clamp Sanitary Hygienic Ball Butterfly Check Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PE Biodegradable Sheet Product Line/Single Screw Extruder]

Whitaker,J.R.(1963),Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. Analytical Chemistry35(12), 1950-1953[Wheel-Type-Carriage Precision Heavy Duty Linear Guide]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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