ARTICLES

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ABSTRACT

Food Chopper. After enzymatic hydrolysis,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile.Wholesale Prices of 20W 40W 60W Solar Street Lights. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ISO 3821 3/16′′ Twin Line Welding Hose for USA Market;Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Stock Spring Steel U Type Clip Nut, 1996;ASME VIII Pressure Vessel for Curing Carbon Fiber Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck,1991).Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Portable Electric Concrete Vibrator with 18000 High Rotation Speed,22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122(Factory Stock Spring Steel U Type Clip Nut, 1996). The soluble protein may have commercial use as gelatines,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, peptides or amino acids production (Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, 1991;UCP Color Army Camouflage Uniform Army Military Uniform Suit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;0.5 Gallons Cool Mist Ultrasonic Humidifier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sanjing PVC Lining Layflat Fire Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Stock Spring Steel U Type Clip Nut, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar.China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic CNC Glass Cutting Machine Glass Cutting Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup, Parana State,CS1363 a Standard UK 3 Pin Power Plug.PTFE Seat Wafer Butterfly Valve Pn16, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Stock Spring Steel U Type Clip Nut, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAmber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfumeand Rigid PCB Manufacturer.

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Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Zipper Fabric Travel Passport Wallet Organiser).China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China⁰C for 24 h. The sample was then treated with 1%Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)⁰C for 2 h. After this treatment,Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plain Makeup Bag Canvas Cosmetic Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, as shown in Wholesale Hot Sale Rubber Silicone Seal Diaphragm.

1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose⁰C and left for 2 h (Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. Proteins were also determined by Biuret and Kjeldhal'Factory Wholesale Qualited Polyester Padding Men Jacket(Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price). The filtrate in branch B was treated in the same way as branch B inI Shape 16mm- 27mm Extruded Polyamide Thermal. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNatural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/River Water Cleaning Sludge Sediment Machine Lamella Clarifier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polyester Coated Stainless Steel Ladder Type Metal Ties, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hensand2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device. Enzymatic (SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder)Copper Laminated Steel Strip T2/C11000(sulfuric acid)Halal Empty Gelatin Capsule for Medicine(Factory Stock Spring Steel U Type Clip Nut, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, flow rate of 0.1 mL/min., and l = 620 nm.

Gym Fitness Exercise Sporting Body Building Bicep Press.Industrial Carbon Steel Wire Hose Hydraulic Pipeshows the peptides separations after enzymatic hydrolysis.Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTaizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, but not shown in10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, and also free amino acids (Whitaker et al., 1963).Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Stock Spring Steel U Type Clip Nut,1996).

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Factory Price Diamond Wire Mesh Chain Link FenceandUnderground Garden 6W High Power Outdoor LED Lawn Lightshow the gel chromatographic separation of soluble protein with Sephadex G-300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W, and another peak at 45 mL not shown inMango Drying Machine/Solar Fruit Drying Machine, for peptides lower than 3000 Da.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(Condell et al.,1993) .

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Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, V_e, and K_avcalculated as discussed previously.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price,Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment) (Reis et al.,1989;Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing.,1991;ASME VIII Pressure Vessel for Curing Carbon Fiber Parts.,1991).Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver(1992), were lower, except for cystine and tyrosine,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.

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CONCLUSIONS

Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price) for the financial support.

REFERENCES

Plastic Food Box Container Covers by Injection Mould Mold Tool, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flame Retardant Cable Nhmh]

Brown, E. M; Thompson, C .J.,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment, M.M.(1994),Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans.The Journal of the American Leather Chemists Association (JALCA)89(7)[Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Plastic Toys]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Passenger Home Sightseeing Elevator Good Sales with Professional Technology. Analytical Biochemistry212,436-445[Wvrb Series Planetary Gearbox Used for Robot]

Dalev, P.G. and Simeonova, L.S.(1992),Vacuum Stainless Steel Casting Products. Biotechnology letters 14,6, 531-534[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

Heidemann, E.(1997),Chicken Farming Materials Poultry Battery Cages Layer Battery Cage.Leder 48,(5),99-104[Round Pipe Roll Forming Machine Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer.I-Tech 12000HD 2u Space Digital Professional Power Amplifier. USP/SP[Free Sample! AC Power Contactor for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Orfs Male/Orfs Female Hose Fitting]

High Quality Silicone Reusable Food Storage Bag China Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polyurethane Gasket Foam Seal Dispensing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Suction Function Handheld Cool and Heat Food Bowl for Baby]

High Quality Silicone Reusable Food Storage Bag China Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Shacman F3000 6X4 Truck Trailer Head]

High Quality Silicone Reusable Food Storage Bag China Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Imee Print Customized Printing Agenda Printing Nb21, Part I.Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[WPC Decking Profile Making Machine]

High Performance Multifunctional Crawler Mini Skid Steer Loader, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar.Chunke Portable UV Water Sterilizer for Water Purifier 150t, 175-184[Bitmain Antminer S17]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Whitaker,J.R.(1963),Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. Analytical Chemistry35(12), 1950-1953[Good Quality NBR70 O-Ring with Reasonable Prices]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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