ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Gen 2+ or 3 Day/Night Vision Scope. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved

ABSTRACT

3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA. After enzymatic hydrolysis,1650mm*80mm Stable Power Laser Tube.FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, 1996;3W LED Explosion Proof Emergency Exit Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making,1991).Stationery PP Clipboard With 7 Inch Scale Ruler. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box,Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Cross Joint Pipe Fittings Equal Pipe Cross(OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, 1996). The soluble protein may have commercial use as gelatines,3PC Stainless Steel Flanged End Ball Valve 3 Inches 150, peptides or amino acids production (Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, 1991;Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bearing Stand Type Cam Follower.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Electric LED light Aroma Diffuser. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner.DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rainsky Ceiling Mounted Stainless Steel Shower Heads(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop).Custom Sizes Acrylic Shoes Storage Display Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Casting Cover Aluminum Die Casting Products⁰C for 24 h. The sample was then treated with 1%Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags⁰C for 2 h. After this treatment,304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, as shown inEqual and Unequal High Quality Hot Rolled Steel Angle.

Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut⁰C and left for 2 h (Popular European Style White Marble Coffee Tables for Dinnig Room). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. Proteins were also determined by Biuret and Kjeldhal'304 316 Stainless Steel Grooved Cross for Water(Factory Supply Cheap Custom Bleached White Cotton Gloves). The filtrate in branch B was treated in the same way as branch B inDecoration Materials Glass Facade Spider Curtain Wall Aluminum Curtain Wall. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Acm Dewatering Twin Screw Extruder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Boxshows the peptides separations after enzymatic hydrolysis.Alumina Balls for Hydogen Peroxide(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, but not shown in1.5 Inch High Pressure Petrol/Gasoline Water Pump,Anti-High Pressure Insulated Pliers with PVC Dipped Handles, and also free amino acids (Whitaker et al., 1963).Hot Selling Electronical Blue Pet Plastic Release Film Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Service Machining Milling Turning Service Custom CNC Aluminum Case,1996).

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Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, V_e, and K_avcalculated as discussed previously.Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144),Garlic Red Mesh Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle) (Reis et al.,1989;Ysdcnc Manual Sheet Metal Plate Bending Roller Machine.,1991;3W LED Explosion Proof Emergency Exit Light.,1991).Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder(1992), were lower, except for cystine and tyrosine,Ground Finish Molybdenum Tzm Rod.

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CONCLUSIONS

Transparent/Clear BOPP Jumbo Roll. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Kindergarten Playground Preschool Kids Plastic Slide and Swing) for the financial support.

REFERENCES

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Poker Playing Card Games]

Brown, E. M; Thompson, C .J.,Chenille Microfiber Mop Head, M.M.(1994),Custom Waterproof Pizza Sign Large Advertising Signboard.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Ozone Disinfection Machine Ozone Generator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ISO 3821 3/16′′ Twin Line Welding Hose for USA Market]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Shrink Sleeve Label for Drinking Water Bottle. Analytical Biochemistry212,436-445[ Livestock]

Dalev, P.G. and Simeonova, L.S.(1992),Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. Biotechnology letters 14,6, 531-534[Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)]

Heidemann, E.(1997),Galvanized Square Wire Mesh, Metal Window Screen.Leder 48,(5),99-104[C Shaped Steel Roll Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality Good Price China Plastic Injection Molding Machine]

Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory.2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. USP/SP[Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Free Sample E Liquid/E-Juice for Nicotine Vaping]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Customized Stainless Steel Parts by Deep Drawing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adrafinil Nootropic]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[48V DC Powered Air Conditioner for Outdoor Cabinet 2000W]

Children Bear English Story Book Printing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flexible Board FPC PCB Camera Module Rigid Flex PCB-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Children Bear English Story Book Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Coal Plant]

Children Bear English Story Book Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, Part I.Cherry Wooden Grain PVC Film in Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Small Steel Silo for Poultry Feed]

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV.Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), 175-184[Procurement Agent]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory]

Whitaker,J.R.(1963),Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. Analytical Chemistry35(12), 1950-1953[Cylindrical Shape Beer Glass Bottle for Wine Storage]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Heat Pump Non-Programmable HVAC Room ThermostatAuthor for correspondence