ARTICLES

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ABSTRACT

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. After enzymatic hydrolysis,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.304 &314 Stainless Steel Seamless Bending Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, 1996;Custom Grey Full-Zip Cotton Mens Hoodies., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook,1991).7inch Industrial Tablet Computer Naked Resistance Touch Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glass Door Passenger Sightseeing Elevator for Sale,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor(Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, 1996). The soluble protein may have commercial use as gelatines,Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, peptides or amino acids production (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 1991;Fcy30 Transport Mini Tipping Dumper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Premium Grade Rubber Cloth Duct Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sud1000h Poly Welder Butt Fusion Machine.Customized Anti-Rust Torsion Spring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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27L/Min Water Exchanger Heat Pump Mold Temperature Machine⁰C and left for 2 h (7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Felt Belt for Textile Compactor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. Proteins were also determined by Biuret and Kjeldhal'Metal Soft Enamel Souvenir Metal Medal Sport Medallions(2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)). The filtrate in branch B was treated in the same way as branch B inHigh Quality Stainless Steel Kitchen Sink. The final soluble protein shown were used for peptides separations using gel permeation chromatography,20L Water Tank Lid Mold 3D Printing Samples Available.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale High Quality Metal Car Mount Holder Phone Holder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBiggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pet Laminate Aluminium Foil/Pet+Alu+PE⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Design Multi-Color Silicone Coin Purse for Lady, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic) for the financial support.

REFERENCES

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Reversible Lightweight Quick-Drying Stashable Plain Green Hat Wholesale]

Brown, E. M; Thompson, C .J.,Polyester / Polypropylene Woven Geotextile for Road Construction, M.M.(1994),Granite Stone Jaw Crusher of Mining Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Round Shape All Color Pets Feeding Dog Bowl of China Supplies]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor. Analytical Biochemistry212,436-445[25kg 50kg Plastic PP Woven Feed Bags]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. Biotechnology letters 14,6, 531-534[360 Degree Rotation Medical Seat Cushion]

Heidemann, E.(1997),The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine.Leder 48,(5),99-104[Cold Galvanized Iron Wire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Oak Laminate Grey Floating Cork Vinyl Plank Flooring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2019 Factory 200W 5r Sharpy Beam DJ Disco Stage Equipment Moving Head Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine]

Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, Part I.Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Home Use Standing Modern Floor Lamp (KAF6108)]

Taiding Custom Tank Sheet Metal Fabrication, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Airtight Candy Bin with Scoop.Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar, 175-184[Slide Rail]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts]

Whitaker,J.R.(1963),Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell. Analytical Chemistry35(12), 1950-1953[ 230V]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

160 Short Pitch Precision Roller Chain High Tensile Strength Roller ChainAuthor for correspondence