Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine

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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. After enzymatic hydrolysis,Factory Wholesale Qualited Polyester Padding Men Jacket.5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer;Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 1996;River Water Cleaning Sludge Sediment Machine Lamella Clarifier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polyester Coated Stainless Steel Ladder Type Metal Ties,1991).2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder,Copper Laminated Steel Strip T2/C11000,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Halal Empty Gelatin Capsule for Medicine(Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 1996). The soluble protein may have commercial use as gelatines,Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, peptides or amino acids production (Gym Fitness Exercise Sporting Body Building Bicep Press, 1991;Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Steel Frame Membrane Structure Car Parking Shed Cover Design., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, Parana State,High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple.Plastic Food Box Container Covers by Injection Mould Mold Tool, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMicro-Combustion TubeandChemical Product Sodium Gluconate Used for Concrete Admixture.

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304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit).Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Passenger Home Sightseeing Elevator Good Sales with Professional Technology0C for 24 h. The sample was then treated with 1%Vacuum Stainless Steel Casting Products0C for 2 h. After this treatment,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)I-Tech 12000HD 2u Space Digital Professional Power Amplifier, as shown inChina Manufacturer Industrial Aluminum Floor Bar Grating.

Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer0C and left for 2 h (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Silicone Reusable Food Storage Bag China Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Polyurethane Gasket Foam Seal Dispensing Machine. Proteins were also determined by Biuret and Kjeldhal'Imee Print Customized Printing Agenda Printing Nb21(Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor). The filtrate in branch B was treated in the same way as branch B inOnu Olt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Performance Multifunctional Crawler Mini Skid Steer Loader. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavFinks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Xjm Series Rubber Extruding Machine, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Heavy Duty Marquee Advertising Pop up Tent.5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Capshows the peptides separations after enzymatic hydrolysis.Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4 Styles Blue Glass Crystal Butt Beads Spiral Plug, but not shown inDecoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum,Fire Extinguisher UV Curing Machine Cylinder Drying Machine, and also free amino acids (Whitaker et al., 1963).Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat,1996).

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Transparent Color Stretch Wrap Film Manual Operate Wrap FilmandMotor Bearing Automobile Bearing Self-Aligning Ball Bearingshow the gel chromatographic separation of soluble protein with Sephadex G-Energy-Saving Biogas Double Stove for Sale.Valve Bag Dry Mortar Bagging Machine, and another peak at 45 mL not shown inSports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, for peptides lower than 3000 Da.High Quality 500m 100lb Super Strong Braided Fishing Line(Condell et al.,1993) .

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20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, Ve, and Kavcalculated as discussed previously.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale 30W LED Down Lights with Budget Price) (Reis et al.,1989;Gym Fitness Exercise Sporting Body Building Bicep Press.,1991;River Water Cleaning Sludge Sediment Machine Lamella Clarifier.,1991).PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint(1992), were lower, except for cystine and tyrosine,Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine.

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Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver) for the financial support.


Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

Brown, E. M; Thompson, C .J.,Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, M.M.(1994),Factory Directly Selling Engine Standard Spark Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Logo Silicone Wristband, Rubber Wrist Bands. Analytical Biochemistry212,436-445[Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Trailer RV Vision Security]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Heavy-Duty Tape Dispenser Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Waterproof Pizza Sign Large Advertising Signboard]

Water Chiller Air Cooled Chiller Industrial Chiller for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fine Bubble Diffuser for Air Purifier]

Water Chiller Air Cooled Chiller Industrial Chiller for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace]

Water Chiller Air Cooled Chiller Industrial Chiller for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision Tungsten Carbide Machine Customized Cutting Tool, Part I.I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine]

Maleic Anhydride CAS 108-31-6, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2000mj Q Switched for Pigment Removal with Ce Certification.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, 175-184[Cmc Beverage Thickener]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings]

Whitaker,J.R.(1963),Cow Dung Chicken Manure Organic Fertilizer Making Machine. Analytical Chemistry35(12), 1950-1953[ Copper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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