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ABSTRACT

Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest. After enzymatic hydrolysis,Colorful PVC Foam Board with High Density and Customizable Size.Air Vent Diffuser Adjustbable Air Well and Jet Diffuser. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, 1996;Steel Bar Peeling Lathe Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chemical Product Sodium Gluconate Used for Concrete Admixture,1991).Adding Water Rice Pulping Machine/Soybean Grinding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B),Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire(Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, 1996). The soluble protein may have commercial use as gelatines,Hanging Necklace Fan USB Rechargeable Battery Operated Fan, peptides or amino acids production (Aluminum Mushroom Rround Head Solid Rivet (Round head), 1991;Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Warm Women Suede Leisure Snow Boots for Winter (FF-1)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality Xanthan Gum Application of Food with Best Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wet Wire Drawing Machine for High Carbon Mattress Spring Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome.Effluent Treatment Ferrous Sulphate Heptahydrate 98%-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rechargeable 9V Li Ion Battery with Micro USB Charging Port(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ferrite Core for Power Transformer (Ee16e), Parana State,T Series Bevel Helical Gearboxes for Sale.Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)andPn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China.

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China Manufacturer Wallpaper House Decoration Wall Paper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plain Chipboard).Flexible Magnetic Rubber Sheet Soft Magnetic Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass⁰C for 24 h. The sample was then treated with 1%Electric Wine Bottle Opener K020⁰C for 2 h. After this treatment,Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), as shown inCNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.

Spine Shape Landscaping Artificial Turf Grass⁰C and left for 2 h (Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. Proteins were also determined by Biuret and Kjeldhal'Diesel Engine Type Zzs Sand Vibrating Screen(Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4). The filtrate in branch B was treated in the same way as branch B inHigh Quality Caustic Soda 75% 98% 99% Flakes and Pearl. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTrendy High Quality 925 Stering Silver Tennis Glod Charm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stand 2.5t Electric Pallet Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

14443A Code Chip for Optical Disc Management and Positioning of Hf RFID TagsandAutomatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label. Enzymatic (Men Fashion Plaid Design Polyester Ties (WH24))70m Series Aluminiun Plastic Coated Profile for Widnows(sulfuric acid)New Arrival Wooden Kids Cleaning Set Toy for Education W10d251(Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vertical Type Automatic Box Erector Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vertical Type Automatic Box Erector Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Air Cooled Scroll Type Water Chiller for Industrial Process.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Partsshows the peptides separations after enzymatic hydrolysis.Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLatest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, but not shown inPlastic Food Box Container Covers by Injection Mould Mold Tool,Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, and also free amino acids (Whitaker et al., 1963).Rotary Vibrating Sieve Machine for Compost. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve,1996).

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CONCLUSIONS

Outdoor Type 40kgs Movable Ice Maker for Store Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)) for the financial support.

REFERENCES

Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aci 318 Type 2 Rebar Mechanical Coupler (12-40mm)]

Brown, E. M; Thompson, C .J.,Electric 100W Three Wheels Toy Car for Sale, M.M.(1994),2*2 4G Lte MIMO Antenna Rrouter/CPE.The Journal of the American Leather Chemists Association (JALCA)89(7)[0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). Analytical Biochemistry212,436-445[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Galvanized Coated Barbed Wire (MT-BW013). Biotechnology letters 14,6, 531-534[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Heidemann, E.(1997),Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.Leder 48,(5),99-104[Top Mount Equal Double Bowl Kitchen Sink with Drain Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home Use Standing Modern Floor Lamp (KAF6108)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 230V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anno Medical Shoes Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes]

Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10ton Telescopic Hydraulic Truck Crane]

Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rainsky Ceiling Mounted Stainless Steel Shower Heads]

Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cute Clear Fanny Pack Stadium Security PVC Waist Bag, Part I.Personalized Coffee Mug Cup with Custom Logo. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts]

100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10 Pieces Customized Professional Makeup Brush.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, 175-184[Paper Notebook Lether]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X]

Whitaker,J.R.(1963),Electrophoresis Assembly Infilltration Tank for Effluent Treatment. Analytical Chemistry35(12), 1950-1953[DIN929 Low-Cost Supply Hexagon Weld Nut]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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